Nuclear phosphoinositide breakdown mediated by nuclear PLCbeta phosphorylation is a downstream effect of IL-2 stimulation in primary human NK cells that is involved in the proliferative response to IL-2. Here we investigated whether the nuclear phosphoinositide turnover in NK cells is a response confined to IL-2, or rather represents a more general mechanism of nuclear signalling linked to the proliferative response of NK cells to activatory cytokines. We therefore focused on IL-12 and IL-15-induced nuclear events in primary human NK cells. Our results show that IL-12 and IL-15 activate nuclear PLCbeta1 in NK cells, with delayed kinetics as compared to IL-2. The nuclear PLC activation induced by the cytokines could be blocked by the MEK-1 inhibitor PD 98059, suggesting its dependence upon MAPKinase activation. Our conclusion is that the three cytokines that activate NK cells and that bind partially similar (IL-2, IL-15) or different surface receptors (IL-12), all induce activation of PLCbeta1 in the nucleus of NK cells via MAPKinase. Thus, the activation of nuclear PLCbeta1 appears as a physiologically relevant general mechanism of response to cytokine stimulation in human natural killer cells.
IL-12 and IL-15 induce activation of nuclear PLCbeta in human natural killercells / PONTI C.; FALCONI M.; BILLI AM.; FAENZA I.; CASTORINA S.; CAIMI L.; CACCHIOLI A.; COCCO L.; VITALE M.. - In: INTERNATIONAL JOURNAL OF ONCOLOGY. - ISSN 1019-6439. - 20(1)(2002), pp. 149-153.
|Tipologia ministeriale:||Articolo su rivista|
|Appare nelle tipologie:||1.1 Articolo su rivista|