The involvement of nuclear inositol lipids in the processes related to DNA repair upon ionizing radiation has been investigated in Murine Erythroleukaemia cells. Early changes in the in vitro phosphatidylinositol-bisphosphate phosphorylation in isolated nuclei were found to precede transiently the marked increase in DNA synthesis occurring after irradiation. Such an increase detected by anti-BrdU monoclonal antibodies has been found to be related mainly to DNA polymerase beta activity as revealed by the kinetic analysis of in vitro DNA synthesis. The results here presented allow us to speculate on a possible involvement of nuclear inositol lipids in the cascade of the early events leading to the regulation of DNA repair in the nucleus.

Evidence for an early and transient involvement of nuclear inositol lipids insubcellular signalling events related to DNA repair processes / RANA RA.; CATALDI A.; DI PIETRO R.; MAZZOTTI G.; CENTURIONE L.; ROBUFFO I.; VITALE M.; MISCIA S.. - In: CELLULAR SIGNALLING. - ISSN 0898-6568. - 6(4)(1994), pp. 475-480. [10.1016/0898-6568(94)90095-7]

Evidence for an early and transient involvement of nuclear inositol lipids insubcellular signalling events related to DNA repair processes

VITALE, Marco;
1994

Abstract

The involvement of nuclear inositol lipids in the processes related to DNA repair upon ionizing radiation has been investigated in Murine Erythroleukaemia cells. Early changes in the in vitro phosphatidylinositol-bisphosphate phosphorylation in isolated nuclei were found to precede transiently the marked increase in DNA synthesis occurring after irradiation. Such an increase detected by anti-BrdU monoclonal antibodies has been found to be related mainly to DNA polymerase beta activity as revealed by the kinetic analysis of in vitro DNA synthesis. The results here presented allow us to speculate on a possible involvement of nuclear inositol lipids in the cascade of the early events leading to the regulation of DNA repair in the nucleus.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2430682
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