A multiparametric analysis of the effects of human recombinant interferon alpha type A on Daudi cells involving flow cytometry and in vitro analysis of alpha and beta DNA polymerase activities has been performed. Results have disclosed (within 60 min of interferon treatment) a decrease of alpha polymerase driven DNA synthesis persisting to at least 24 h, while beta polymerase was poorly affected. Moreover, after 24 h of interferon treatment, a reduction of BrdUrd incorporation per cell, assessed by flow cytometry, was observed suggesting that DNA synthesis in S phase cells is almost completely abolished. The analysis of the effect of interferon on the distribution of cell cycle phases indicated that the G1/S transition is not inhibited by the treatment. These results support the hypothesis that interferon generates a transient initiating signal which quickly reaches the nucleus and produces a rapid inhibition of alpha polymerase activity, leading finally to the slowing of cell cycle progression.
Interferon affects cell growth progression by modulating DNA polymerases activity / CATALDI A.; SANTAVENERE E.; VITALE M.; TRUBIANI O.; LISIO R.; TULIPANO G.; DI DOMENICANTONIO L.; ZAMAI L.; MISCIA S.. - In: CELL PROLIFERATION. - ISSN 0960-7722. - 25(3)(1992), pp. 225-231.
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