Chromosomal DNA measurements represent the most accurate methodology for the quantitative analysis of human karyotype. A modified FACS 440 signal collection is described here. The FSC signal voltage coming from the optical bench has been split in two equal parts by a T-connector placed at the preamplifier entrance. The side scatter channel that is not employed during chromosome analysis has been used for the half-FSC signal. This modification allowed the contemporary use of FSC signal both for gating and analysis leading to a better discrimination of background, and therefore to a more accurate definition of sorting windows.
Technical improvements for flow karyotyping by standard FACS 440 flow cytometer / VITALE M.; ZAMAI L.; ANTONUCCI A.; DEL GRANDE P.; PAPA S.. - In: CYTOTECHNOLOGY. - ISSN 0920-9069. - 5(3)(1991), pp. 195-199.
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