Objective - This field trial was aimed at assessing the immune efficacy of the concurrent use of PCV2 + PRRS vaccinations in comparison with the effects of both single vaccinations (PRRS and PCV2 alone) and with negative non-vaccinated control pigs by measuring IFN-γ-specific SC. Materials and Methods - This double-blinded trial was carried out in a farrow to finish 2000-sow-herd affected by PCVD and infected by PRRSV in nursery and growing phase (stable herds). PCV2 and PRRS vaccinations have been never applied prior to this trial. At weaning (22.5±1.3 days of age) 800 piglets individually identified were equally distributed to 4 different groups as follow: A (concurrent vaccination with Porcilis PRRS - right side of the neck, + Porcilis PCV - left side); B (Porcilis PRRS only); C ( Porcilis PCV only); D (Placebo -adjuvant/control). Blood samples (20 pigs/group) were taken every 2 weeks for the whole duration of the trial in order to perform immunological exams to evaluate specific immune response to PCV2 and PRRSV. PRRS natural infection occurred from 8 to 18 weeks of age and PCV2 infection from 16 weeks of age onward. Data were statistically evaluated by using ANOVA (analysis of variance) and Dunnett’s test. Results - After PCV2 vaccination both vaccinated groups (A and C) developed an increase of the number of IFN-g SC. Conversely, in non PCV2 vaccinated groups the amount of SC stayed at basal level. After infection all groups showed an increase of IFN-g SC especially in concurrently vaccinated pigs. After PRRS vaccination the number of IFN-g SC in concurrently vaccinated and group B increased promptly reaching higher level than the non-vaccinated groups. Conclusions - In the condition of this study, the results show that concurrent vaccination to PRRS and PCV2 is safe and does not interfere with the stimulation of the cell mediated immunity to the specific antigen and shows some improvement of cell mediated response to PCV2.
STUDY ON CELL MEDIATED IMMUNE RESPONSE INDUCED BY CONCURRENT VACCINATIONS TO PRRSV AND PCV2 IN NATURALLY INFECTED PIGS / Ferrari, Luca; DE ANGELIS, Elena; Morganti, Marina; Saleri, Roberta; Cavalli, V.; Ardigo', P.; Guazzetti, S.; Borghetti, Paolo; Martelli, Paolo. - (2012), pp. 154-154. (Intervento presentato al convegno 4TH EUROPEAN SYMPOSIUM ON PORCINE HEALTH MANAGEMENT tenutosi a BRUGES nel 25-27 APRILE 2012).
STUDY ON CELL MEDIATED IMMUNE RESPONSE INDUCED BY CONCURRENT VACCINATIONS TO PRRSV AND PCV2 IN NATURALLY INFECTED PIGS
FERRARI, Luca;DE ANGELIS, Elena;MORGANTI, Marina;SALERI, Roberta;BORGHETTI, Paolo;MARTELLI, Paolo
2012-01-01
Abstract
Objective - This field trial was aimed at assessing the immune efficacy of the concurrent use of PCV2 + PRRS vaccinations in comparison with the effects of both single vaccinations (PRRS and PCV2 alone) and with negative non-vaccinated control pigs by measuring IFN-γ-specific SC. Materials and Methods - This double-blinded trial was carried out in a farrow to finish 2000-sow-herd affected by PCVD and infected by PRRSV in nursery and growing phase (stable herds). PCV2 and PRRS vaccinations have been never applied prior to this trial. At weaning (22.5±1.3 days of age) 800 piglets individually identified were equally distributed to 4 different groups as follow: A (concurrent vaccination with Porcilis PRRS - right side of the neck, + Porcilis PCV - left side); B (Porcilis PRRS only); C ( Porcilis PCV only); D (Placebo -adjuvant/control). Blood samples (20 pigs/group) were taken every 2 weeks for the whole duration of the trial in order to perform immunological exams to evaluate specific immune response to PCV2 and PRRSV. PRRS natural infection occurred from 8 to 18 weeks of age and PCV2 infection from 16 weeks of age onward. Data were statistically evaluated by using ANOVA (analysis of variance) and Dunnett’s test. Results - After PCV2 vaccination both vaccinated groups (A and C) developed an increase of the number of IFN-g SC. Conversely, in non PCV2 vaccinated groups the amount of SC stayed at basal level. After infection all groups showed an increase of IFN-g SC especially in concurrently vaccinated pigs. After PRRS vaccination the number of IFN-g SC in concurrently vaccinated and group B increased promptly reaching higher level than the non-vaccinated groups. Conclusions - In the condition of this study, the results show that concurrent vaccination to PRRS and PCV2 is safe and does not interfere with the stimulation of the cell mediated immunity to the specific antigen and shows some improvement of cell mediated response to PCV2.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
