Abstract L-Methionine sulfoximine (MSO) and DL-Phosphinothricin (PPT), two non-proteinogenic amino acids known as inhibitors of Glutamine Synthetase, cause a dosedependent increase in the phosphorylation of the mTOR substrate S6 kinase 1. The effect is particularly evident in glutamine-depleted cells, where mTOR activity is very low, but is detectable for PPT also in the presence of glutamine. The stimulation of mTOR activity by either MSO or PPT is strongly synergized by essential amino acids. Thus, the non-proteinogenic amino acids MSO and PPT are mTOR activators.
The non-proteinogenic amino acids L-methionine sulfoximine and DL-phosphinothricin activate mTOR / Tardito, S.; Chiu, M.; Franchi, Renata; Dall'Asta, Valeria; Comi, P.; Bussolati, Ovidio. - In: AMINO ACIDS. - ISSN 0939-4451. - 42:(2012), pp. 2507-2507. [10.1007/s00726-011-0981-4]
The non-proteinogenic amino acids L-methionine sulfoximine and DL-phosphinothricin activate mTOR
M. Chiu;FRANCHI, Renata;DALL'ASTA, Valeria;BUSSOLATI, Ovidio
2012-01-01
Abstract
Abstract L-Methionine sulfoximine (MSO) and DL-Phosphinothricin (PPT), two non-proteinogenic amino acids known as inhibitors of Glutamine Synthetase, cause a dosedependent increase in the phosphorylation of the mTOR substrate S6 kinase 1. The effect is particularly evident in glutamine-depleted cells, where mTOR activity is very low, but is detectable for PPT also in the presence of glutamine. The stimulation of mTOR activity by either MSO or PPT is strongly synergized by essential amino acids. Thus, the non-proteinogenic amino acids MSO and PPT are mTOR activators.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
