The feasibility of a DNA biosensor based on a long period grating inscribed in an optical fiber has been investigated. The sensor can perform a label-free detection, due to the functionalization of the optical fiber external surface with peptide nucleic acid probes matched with the DNA target strands. A wavelength shift of 1.2 nm for a 120 nM DNA solution has been measured. Fluorescence measurements have been also taken, in order to give a further proof of the fiber functionalization and hybridization.
Long period grating-based fiber optic sensor for label-free DNA detection / Sozzi, Michele; Coscelli, Enrico; Poli, Federica; Cucinotta, Annamaria; Corradini, Roberto; Marchelli, Rosangela; M., Konstantaki; S., Pissadakis; Selleri, Stefano. - (2011), pp. 1-3. (Intervento presentato al convegno 2011 International Workshop on Biophotonics tenutosi a Parma, Italy nel June 8-10, 2011) [10.1109/IWBP.2011.5954847].
Long period grating-based fiber optic sensor for label-free DNA detection
SOZZI, MICHELE;COSCELLI, Enrico;POLI, Federica;CUCINOTTA, Annamaria;CORRADINI, Roberto;MARCHELLI, Rosangela;SELLERI, Stefano
2011-01-01
Abstract
The feasibility of a DNA biosensor based on a long period grating inscribed in an optical fiber has been investigated. The sensor can perform a label-free detection, due to the functionalization of the optical fiber external surface with peptide nucleic acid probes matched with the DNA target strands. A wavelength shift of 1.2 nm for a 120 nM DNA solution has been measured. Fluorescence measurements have been also taken, in order to give a further proof of the fiber functionalization and hybridization.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
