Abstract The ORF50/Rta gene has been shown to be an essential gene for many gammaherpesviruses. Although the BoHV-4 ORF50/Rta homolog, immediate early gene 2 (IE2), has been shown to activate several BoHV-4 early and late promoters in cotransfection assays, there is no direct proof of its indispensability for progression of the virus to the lytic replication cycle in the context of the viral genome. In the present communication, replication defective BoHV-4-V.test IE2 mutants were efficiently rescued, with respect to production of infectious virus and DNA replication, upon the expression of BoHV-4 ORF50/Rta in trans. Surprisingly, in the course of our studies, we discovered that the IE2 gene is duplicated in the genome of BoHV-4-U. Keywords: Bovine herpesvirus 4; Immediate early gene; Gene duplication
Bovine herpesvirus 4 immediate early 2 (Rta) gene is an essential gene and is duplicated in bovine herpesvirus 4 isolate U / Franceschi, Valentina; Capocefalo, Antonio; Ravanetti, L; Vanderplasschen, A; Gillet, L; Cavirani, Sandro; van Santen, Vl; Donofrio, Gaetano. - In: VETERINARY MICROBIOLOGY. - ISSN 0378-1135. - 148:(2011), pp. 219-231. [10.1016/j.vetmic.2010.09.031]
Bovine herpesvirus 4 immediate early 2 (Rta) gene is an essential gene and is duplicated in bovine herpesvirus 4 isolate U.
FRANCESCHI, Valentina;CAPOCEFALO, Antonio;CAVIRANI, Sandro;DONOFRIO, Gaetano
2011-01-01
Abstract
Abstract The ORF50/Rta gene has been shown to be an essential gene for many gammaherpesviruses. Although the BoHV-4 ORF50/Rta homolog, immediate early gene 2 (IE2), has been shown to activate several BoHV-4 early and late promoters in cotransfection assays, there is no direct proof of its indispensability for progression of the virus to the lytic replication cycle in the context of the viral genome. In the present communication, replication defective BoHV-4-V.test IE2 mutants were efficiently rescued, with respect to production of infectious virus and DNA replication, upon the expression of BoHV-4 ORF50/Rta in trans. Surprisingly, in the course of our studies, we discovered that the IE2 gene is duplicated in the genome of BoHV-4-U. Keywords: Bovine herpesvirus 4; Immediate early gene; Gene duplicationI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
