Development of the human gut microbiota commences at birth, with bifidobacteria being among the first colonizers of the sterile newborn gastrointestinal tract. To date, the genetic basis of Bifidobacterium colonization and persistence remains poorly understood. Transcriptome analysis of the Bifidobacterium breve UCC2003 2.42-Mb genome in a murine colonization model revealed differential expression of a type IVb tight adherence (Tad) pilus-encoding gene cluster designated "tad(2003)." Mutational analysis demonstrated that the tad(2003) gene cluster is essential for efficient in vivo murine gut colonization, and immunogold transmission electron microscopy confirmed the presence of Tad pili at the poles of B. breve UCC2003 cells. Conservation of the Tad pilus-encoding locus among other B. breve strains and among sequenced Bifidobacterium genomes supports the notion of a ubiquitous pili-mediated host colonization and persistence mechanism for bifidobacteria

Functional genome analysis of Bifidobacterium breve UCC2003 reveals type IVb tight adherence (Tad) pili as an essential and conserved host-colonization factor / M., O'Connell Motherway; A., Zomer; S., Leahy; J., Reunanen; F., Bottacini; M. J., Claesson; F., O’Brien; K., O’Flynn; P. C., Casey; J., Moreno Munoz; B., Kearney; B., Houston; C., O’Mahony; D. G., Higgins; F., Shanahan; A., Palva; W., de Vos; G. F., Fitzgerald; Ventura, Marco; P. O., O’Toole; D., van Sinderen. - In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. - ISSN 1091-6490. - 108:(2011), pp. 11217-11222. [10.1073/pnas.1105380108]

Functional genome analysis of Bifidobacterium breve UCC2003 reveals type IVb tight adherence (Tad) pili as an essential and conserved host-colonization factor

VENTURA, Marco;
2011-01-01

Abstract

Development of the human gut microbiota commences at birth, with bifidobacteria being among the first colonizers of the sterile newborn gastrointestinal tract. To date, the genetic basis of Bifidobacterium colonization and persistence remains poorly understood. Transcriptome analysis of the Bifidobacterium breve UCC2003 2.42-Mb genome in a murine colonization model revealed differential expression of a type IVb tight adherence (Tad) pilus-encoding gene cluster designated "tad(2003)." Mutational analysis demonstrated that the tad(2003) gene cluster is essential for efficient in vivo murine gut colonization, and immunogold transmission electron microscopy confirmed the presence of Tad pili at the poles of B. breve UCC2003 cells. Conservation of the Tad pilus-encoding locus among other B. breve strains and among sequenced Bifidobacterium genomes supports the notion of a ubiquitous pili-mediated host colonization and persistence mechanism for bifidobacteria
2011
Functional genome analysis of Bifidobacterium breve UCC2003 reveals type IVb tight adherence (Tad) pili as an essential and conserved host-colonization factor / M., O'Connell Motherway; A., Zomer; S., Leahy; J., Reunanen; F., Bottacini; M. J., Claesson; F., O’Brien; K., O’Flynn; P. C., Casey; J., Moreno Munoz; B., Kearney; B., Houston; C., O’Mahony; D. G., Higgins; F., Shanahan; A., Palva; W., de Vos; G. F., Fitzgerald; Ventura, Marco; P. O., O’Toole; D., van Sinderen. - In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. - ISSN 1091-6490. - 108:(2011), pp. 11217-11222. [10.1073/pnas.1105380108]
File in questo prodotto:
File Dimensione Formato  
pnas.201105380.pdf

non disponibili

Tipologia: Documento in Post-print
Licenza: Creative commons
Dimensione 692.53 kB
Formato Adobe PDF
692.53 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2350575
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 322
  • ???jsp.display-item.citation.isi??? 302
social impact