Mupirocin is an antibiotic commonly used in selective media for the isolation of bifidobacteria. However, little is known about the genetic traits responsible for bifidobacterial resistance to mupirocin. Our investigation demonstrates that all of the bifidobacteria tested exhibit a phenotype of generally high resistance to this antibiotic. The genotypic reason for bifidobacterial mupirocin resistance was further characterized by sequencing of the isoleucyl-tRNA synthetase gene (ileS) coupled with three-dimensional modeling of the encoded protein and cloning of the ileS gene of Bifidobacterium bifidum PRL2010 in a mupirocin-sensitive Escherichia coli strain. These analyses revealed key amino acid residues of the IleS protein that apparently are crucial for conferring a mupirocin resistance phenotype to bifidobacteria

Investigation of mupirocin susceptibility in bifidobacteria: physiological and genetic insights / Serafini, Fausta; F., Bottacini; A., Viappiani; Baruffini, Enrico; Turroni, Francesca; E., Foroni; Lodi, Tiziana; D., van Sinderen; Ventura, Marco. - In: APPLIED AND ENVIRONMENTAL MICROBIOLOGY. - ISSN 0099-2240. - 77:(2011), pp. 3141-3146. [10.1128/AEM.02540-10]

Investigation of mupirocin susceptibility in bifidobacteria: physiological and genetic insights.

SERAFINI, Fausta;BARUFFINI, Enrico;TURRONI, FRANCESCA;LODI, Tiziana;VENTURA, Marco
2011-01-01

Abstract

Mupirocin is an antibiotic commonly used in selective media for the isolation of bifidobacteria. However, little is known about the genetic traits responsible for bifidobacterial resistance to mupirocin. Our investigation demonstrates that all of the bifidobacteria tested exhibit a phenotype of generally high resistance to this antibiotic. The genotypic reason for bifidobacterial mupirocin resistance was further characterized by sequencing of the isoleucyl-tRNA synthetase gene (ileS) coupled with three-dimensional modeling of the encoded protein and cloning of the ileS gene of Bifidobacterium bifidum PRL2010 in a mupirocin-sensitive Escherichia coli strain. These analyses revealed key amino acid residues of the IleS protein that apparently are crucial for conferring a mupirocin resistance phenotype to bifidobacteria
Investigation of mupirocin susceptibility in bifidobacteria: physiological and genetic insights / Serafini, Fausta; F., Bottacini; A., Viappiani; Baruffini, Enrico; Turroni, Francesca; E., Foroni; Lodi, Tiziana; D., van Sinderen; Ventura, Marco. - In: APPLIED AND ENVIRONMENTAL MICROBIOLOGY. - ISSN 0099-2240. - 77:(2011), pp. 3141-3146. [10.1128/AEM.02540-10]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2350573
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