The transcription start site (TSS) is useful to predict gene and to understand transcription initiation. Although vast data on mRNA TSSs are available, little is known about tRNA genes because of rapid processing. Using a tobacco in vitro transcription system under conditions of impaired 5' end processing. TSSs were determined for 64 Arabidopsis tRNA genes. This analysis revealed multiple TSSs distributed in a region from 10 to 2 bp upstream of the mature tRNA coding sequence (-10 to -2). We also analyzed 31 Saccharomyces cerevisiae tRNA genes that showed a smaller number but a broader distribution (-13 to 1) of TSSs. In both cases, transcription was initiated preferentially at adenosine, and a common TCAACA' sequence was found spanning the TSSs. In plant, this motif caused multiple TSSs to converge at one site and enhanced transcription. The TATA-like sequence upstream of Arabidopsis tRNA genes also contributed to TSS selection.
A common sequence motif involved in selection of transcription start sites of Arabidopsis and budding yeast tRNA genes / Y., Yukawa; Dieci, Giorgio; M., Alzapiedi; A., Hiraga; K., Hirai; Y. Y., Yamamoto; M., Sugiura. - In: GENOMICS. - ISSN 0888-7543. - 97:(2011), pp. 166-172. [10.1016/j.ygeno.2010.12.001]
A common sequence motif involved in selection of transcription start sites of Arabidopsis and budding yeast tRNA genes
DIECI, Giorgio;
2011-01-01
Abstract
The transcription start site (TSS) is useful to predict gene and to understand transcription initiation. Although vast data on mRNA TSSs are available, little is known about tRNA genes because of rapid processing. Using a tobacco in vitro transcription system under conditions of impaired 5' end processing. TSSs were determined for 64 Arabidopsis tRNA genes. This analysis revealed multiple TSSs distributed in a region from 10 to 2 bp upstream of the mature tRNA coding sequence (-10 to -2). We also analyzed 31 Saccharomyces cerevisiae tRNA genes that showed a smaller number but a broader distribution (-13 to 1) of TSSs. In both cases, transcription was initiated preferentially at adenosine, and a common TCAACA' sequence was found spanning the TSSs. In plant, this motif caused multiple TSSs to converge at one site and enhanced transcription. The TATA-like sequence upstream of Arabidopsis tRNA genes also contributed to TSS selection.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
