Persistent infection of macrophages with bovine herpesvirus 4 (BoHV-4) has been proposed to play a secondary causal role, along with bacterial infection, in bovine post-partum metritis. Mechanisms of maintenance of BoHV-4 persistent infection are not understood. We previously generated in vitro models of BoHV-4 persistent infection in human rhadomyosarcoma and bovine macrophage cell lines by drug selection of cells infected with BoHV-4 carrying a drug-resistance marker, and demonstrated circular episomal BoHV-4 genomes. In the present study, we used fluorescent in situ hybridization (FISH) to demonstrate BoHV-4 genomes also integrated into the genomes of these persistently infected cells.

Integration of bovine herpesvirus 4 genome into cultured persistently infected host cell genome / Donofrio, Gaetano; Capocefalo, Antonio; Franceschi, Valentina; De Lorenzi, L; van Santen, V; Parma, P.. - In: VIROLOGY JOURNAL. - ISSN 1743-422X. - 7:(2010), pp. 1-4. [10.1186/1743-422X-7-246.]

Integration of bovine herpesvirus 4 genome into cultured persistently infected host cell genome.

DONOFRIO, Gaetano;CAPOCEFALO, Antonio;FRANCESCHI, Valentina;
2010-01-01

Abstract

Persistent infection of macrophages with bovine herpesvirus 4 (BoHV-4) has been proposed to play a secondary causal role, along with bacterial infection, in bovine post-partum metritis. Mechanisms of maintenance of BoHV-4 persistent infection are not understood. We previously generated in vitro models of BoHV-4 persistent infection in human rhadomyosarcoma and bovine macrophage cell lines by drug selection of cells infected with BoHV-4 carrying a drug-resistance marker, and demonstrated circular episomal BoHV-4 genomes. In the present study, we used fluorescent in situ hybridization (FISH) to demonstrate BoHV-4 genomes also integrated into the genomes of these persistently infected cells.
2010
Integration of bovine herpesvirus 4 genome into cultured persistently infected host cell genome / Donofrio, Gaetano; Capocefalo, Antonio; Franceschi, Valentina; De Lorenzi, L; van Santen, V; Parma, P.. - In: VIROLOGY JOURNAL. - ISSN 1743-422X. - 7:(2010), pp. 1-4. [10.1186/1743-422X-7-246.]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2329508
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