Persistent infection of macrophages with bovine herpesvirus 4 (BoHV-4) has been proposed to play a secondary causal role, along with bacterial infection, in bovine post-partum metritis. Mechanisms of maintenance of BoHV-4 persistent infection are not understood. We previously generated in vitro models of BoHV-4 persistent infection in human rhadomyosarcoma and bovine macrophage cell lines by drug selection of cells infected with BoHV-4 carrying a drug-resistance marker, and demonstrated circular episomal BoHV-4 genomes. In the present study, we used fluorescent in situ hybridization (FISH) to demonstrate BoHV-4 genomes also integrated into the genomes of these persistently infected cells.
Integration of bovine herpesvirus 4 genome into cultured persistently infected host cell genome / Donofrio, Gaetano; Capocefalo, Antonio; Franceschi, Valentina; De Lorenzi, L; van Santen, V; Parma, P.. - In: VIROLOGY JOURNAL. - ISSN 1743-422X. - 7:(2010), pp. 1-4. [10.1186/1743-422X-7-246.]
Integration of bovine herpesvirus 4 genome into cultured persistently infected host cell genome.
DONOFRIO, Gaetano;CAPOCEFALO, Antonio;FRANCESCHI, Valentina;
2010-01-01
Abstract
Persistent infection of macrophages with bovine herpesvirus 4 (BoHV-4) has been proposed to play a secondary causal role, along with bacterial infection, in bovine post-partum metritis. Mechanisms of maintenance of BoHV-4 persistent infection are not understood. We previously generated in vitro models of BoHV-4 persistent infection in human rhadomyosarcoma and bovine macrophage cell lines by drug selection of cells infected with BoHV-4 carrying a drug-resistance marker, and demonstrated circular episomal BoHV-4 genomes. In the present study, we used fluorescent in situ hybridization (FISH) to demonstrate BoHV-4 genomes also integrated into the genomes of these persistently infected cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
