Abstract VP2 is the outermost Bluetongue virus (BTV) antigenic protein, forming triskelion motifs on the virion surface. Although VP2 has been expressed successfully through many systems, its paracrine expression as a soluble form by mammalian cells represents a difficult task. In the present paper two fragments of VP2 have been expressed successfully into the medium of transiently transfected mammalian cells through a fusion peptides strategy. The crude conditioned medium containing the secreted peptide could be employed for immunodiagnostic assay development or vaccine purposes.
Expression and secretion of Bluetongue virus serotype 8 (BTV-8)VP2 outer capsid protein by mammalian cells / Capocefalo, Antonio; Franceschi, Valentina; Mertens, Pp; Castillo Olivares, J; Cavirani, Sandro; Di Lonardo, E; Leni, Z; Donofrio, Gaetano. - In: JOURNAL OF VIROLOGICAL METHODS. - ISSN 0166-0934. - 169:(2010), pp. 420-424. [10.1016/j.jviromet.2010.08.002]
Expression and secretion of Bluetongue virus serotype 8 (BTV-8)VP2 outer capsid protein by mammalian cells.
CAPOCEFALO, Antonio;FRANCESCHI, Valentina;CAVIRANI, Sandro;DONOFRIO, Gaetano
2010-01-01
Abstract
Abstract VP2 is the outermost Bluetongue virus (BTV) antigenic protein, forming triskelion motifs on the virion surface. Although VP2 has been expressed successfully through many systems, its paracrine expression as a soluble form by mammalian cells represents a difficult task. In the present paper two fragments of VP2 have been expressed successfully into the medium of transiently transfected mammalian cells through a fusion peptides strategy. The crude conditioned medium containing the secreted peptide could be employed for immunodiagnostic assay development or vaccine purposes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
