Human processed lipoaspirate is a source of multipotent adult stem cells [Strem, B.M., Hicok, K.C., Zhu, M., Wulur, I., Alfonso, Z., Schreiber, R.E., Fraser, J.K., Hedrick, M.H., 2005. Multipotential diVerentiation of adipose tissue-derived stem cells. Keio Journal of Medicine 54 (3), 132–141] that are able to diVerentiate between mesenchymal and neurogenic lineage. We characterized PLA cells by cytometry and then they were cultured to induce diVerentiation into myogenic and neurogenic lineage. Lipoaspirates were digested with collagenase to obtain the pellet, which was labelled with anti-CD44, anti-CD45, and anti-CD90. We used BD FACS Calibur Xow cytometer to acquire cellular events. Some cells were cultured at 37 °C and 5% CO2 in neurogenic or myogenic medium and analysed by immunocytochemistry, using Neuron speciWc enolase, Vimentin, Glial Wbrillary acidic protein, Tau, MAP2 to conWrm neurogenic diVerentiation, MyoD1, Myosin heavy chain, Actin smooth muscle, vimentin to conWrm myogenic diVerentiation. The cytometry results suggest that a part of the cells are of a mesenchymal origin, among which there are progenitor endothelial cells and leucocytes. Microscopy observation already reveals neuronal morphology and longitudinal multinucleated cells compared to control cells. Neurogenic cells only express NSE (early neuronal progenitor marker), but not GFAP, Tau, MAP2 (mature neuron and glial markers); myogenic cells are positive for MyoD1 and Myosin heavy chain. This demonstrates that lipoaspirate cells are capable of diVerentiating in vitro over a short period of time, and could be employed in biological and clinical research, like mesenchymal adult stem cells.
Characterization and induction of human pre-adipocytes / Raposio, E; Guida, C; Baldelli, I; Benvenuto, F; Curto, M; Paleari, L; Filippi, F; Fiocca, R; Robello, G; Santi, P.. - In: TOXICOLOGY IN VITRO. - ISSN 0887-2333. - 21:2(2007), pp. 330-334. [10.1016/j.tiv.2006.09.022]