In this study we have combined fluorescence- and reflection-confocal laser scanning microscopy for the simultaneous visualization of living cells and surface topography beneath them. To this purpose we have designed a specific flow chamber and we have tested it with osteoblasts grown on an opaque, thick support, made of smooth or sandblasted titanium. Cells were loaded with Calcein-AM or tetramethylrhodamine methyl ester (TMRM), two probes employed as indicators of cell viability/morphology and mitochondrial membrane potential, respectively. Besides the acquisition of stacks of confocal sections, the system allowed also vertical views and faithful three-dimensional reconstruction of the samples. Confocal microscope implemented with our flow chamber proved to be a promising tool for time-lapse investigation of cell-biomaterial interactions. © 2007

Analysis of living cells grown on different titanium surfaces by time-lapse confocal microscopy / GATTI R; ORLANDINI G; UGGERI J; BELLETTI S; GALLI C; RASPANTI M; SCANDROGLIO R; GUIZZARDI S.. - In: MICRON. - ISSN 0968-4328. - 39(2008), pp. 137-143. [10.1016/j.micron.2006.11.009]

Analysis of living cells grown on different titanium surfaces by time-lapse confocal microscopy

GATTI, Rita;ORLANDINI, Guido;UGGERI, Jacopo;BELLETTI, Silvana;GALLI, Carlo;SCANDROGLIO, Renato;GUIZZARDI, Stefano
2008

Abstract

In this study we have combined fluorescence- and reflection-confocal laser scanning microscopy for the simultaneous visualization of living cells and surface topography beneath them. To this purpose we have designed a specific flow chamber and we have tested it with osteoblasts grown on an opaque, thick support, made of smooth or sandblasted titanium. Cells were loaded with Calcein-AM or tetramethylrhodamine methyl ester (TMRM), two probes employed as indicators of cell viability/morphology and mitochondrial membrane potential, respectively. Besides the acquisition of stacks of confocal sections, the system allowed also vertical views and faithful three-dimensional reconstruction of the samples. Confocal microscope implemented with our flow chamber proved to be a promising tool for time-lapse investigation of cell-biomaterial interactions. © 2007
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2296004
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