The toxic effects of exogenous hydrogen sulfide on peripheral blood lymphocytes have been investigated in detail. Hydrogen sulfide is now considered as a gasotransmitter with specific functional roles in different cell types, like neurons and vascular smooth muscle. Here we show that exogenous hydrogen sulfide induces a caspase-independent cell death of peripheral blood lymphocytes that depends on their intracellular glutathione levels, with a physiologically relevant subset specificity for CD8+ T cells and NK cells. Although lymphocyte activation does not modify their sensitivity to HS-, after 24 h exposure to hydrogen sulfide surviving lymphocyte subsets show a dramatically decreased proliferation in response to mitogens and a reduced IL-2 production. Overall, our data demonstrate that HS- reduces the cellular cytotoxic response of peripheral blood lymphocytes as well as their production of IL-2, therefore de-activating the major players of local inflammatory responses, adding new basic knowledge to the clinically well known anti-inflammatory effects of sulfur compounds.
Exogenous hydrogen sulfide induces functional inhibition and cell death of cytotoxic lymphocytes subsets / Mirandola, Prisco; Gobbi, Giuliana; Sponzilli, Ivonne; Pambianco, M.; Malinverno, C.; Cacchioli, Antonio; DE PANFILIS, Giuseppe; Vitale, Marco. - In: JOURNAL OF CELLULAR PHYSIOLOGY. - ISSN 0021-9541. - 213(3):(2007), pp. 826-833. [10.1002/jcp.21151]
Exogenous hydrogen sulfide induces functional inhibition and cell death of cytotoxic lymphocytes subsets.
MIRANDOLA, Prisco;GOBBI, Giuliana;SPONZILLI, Ivonne;CACCHIOLI, Antonio;DE PANFILIS, Giuseppe;VITALE, Marco
2007-01-01
Abstract
The toxic effects of exogenous hydrogen sulfide on peripheral blood lymphocytes have been investigated in detail. Hydrogen sulfide is now considered as a gasotransmitter with specific functional roles in different cell types, like neurons and vascular smooth muscle. Here we show that exogenous hydrogen sulfide induces a caspase-independent cell death of peripheral blood lymphocytes that depends on their intracellular glutathione levels, with a physiologically relevant subset specificity for CD8+ T cells and NK cells. Although lymphocyte activation does not modify their sensitivity to HS-, after 24 h exposure to hydrogen sulfide surviving lymphocyte subsets show a dramatically decreased proliferation in response to mitogens and a reduced IL-2 production. Overall, our data demonstrate that HS- reduces the cellular cytotoxic response of peripheral blood lymphocytes as well as their production of IL-2, therefore de-activating the major players of local inflammatory responses, adding new basic knowledge to the clinically well known anti-inflammatory effects of sulfur compounds.File | Dimensione | Formato | |
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