Because food ingredients are sometimes considered as causative factors in IgE mediated food allergies, DNA-based tests may prove to be very useful to establish whether allergenic species have been used in foodstuffs production. The development of two SYBRGreenERTM Real-Time PCR assays, targeting Pru1 and rbcL genes, based on melting curve analysis, to detect allergen species in food has been presented. Applicability of these methods was assessed with several commercial products containing processed almond.
SYBR GREENER Real-Time PCR to detect almond in traces in processed food / Pafundo, Simona; Gulli', Mariolina; Marmiroli, Nelson. - In: FOOD CHEMISTRY. - ISSN 0308-8146. - 116:(2009), pp. 811-815. [10.1016/j.foodchem.2009.03.040]
SYBR GREENER Real-Time PCR to detect almond in traces in processed food
PAFUNDO, Simona;GULLI', Mariolina;MARMIROLI, Nelson
2009-01-01
Abstract
Because food ingredients are sometimes considered as causative factors in IgE mediated food allergies, DNA-based tests may prove to be very useful to establish whether allergenic species have been used in foodstuffs production. The development of two SYBRGreenERTM Real-Time PCR assays, targeting Pru1 and rbcL genes, based on melting curve analysis, to detect allergen species in food has been presented. Applicability of these methods was assessed with several commercial products containing processed almond.| File | Dimensione | Formato | |
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AbstractFC2009.pdf
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