T cells are present in normal adult human skin, but their occurrence in fetal skin is unknown. T cell and Langerhans cell (LC) populations were studied using single or double immunohistochemical staining on cryostat-section. Skin samples taken from different body regions of 17 fetuses ranging from 18 to 30 weeks estimated gestational-age (w-EGA), were examined. In all specimens but one, we did not find any epidermal T cell. In contrast, dermal CD3(+) T cells occurred at all w-EGA. The density of these cells increased with increasing age. Double staining showed that CD3(+) T cells were predominantly CD4(+)/CD45RA(+). On the other hand, LC, as assessed by CD1a expression, was evenly distributed within the interfollicular epidermis and papillary dermis at all gestational ages. Analysis of T cell and LC density in different body regions did not show significant topographic differences. We suggest that lack of epidermal T cells, although the LC network was fully represented, might reflect the scarce opportunity of fetal LC to contact foreign antigens in utero.

Densities, distribution and phenotypic expression of T cells in human fetal skin / DI NUZZO, Sergio; Pavanello, P; Masotti, A; Giordano, Giovanna; DE PANFILIS, Giuseppe. - In: ARCHIVES OF DERMATOLOGICAL RESEARCH. - ISSN 0340-3696. - 301:10(2009), pp. 753-755. [10.1007/s00403-009-0943-9]

Densities, distribution and phenotypic expression of T cells in human fetal skin

DI NUZZO, Sergio
;
GIORDANO, Giovanna;DE PANFILIS, Giuseppe
2009-01-01

Abstract

T cells are present in normal adult human skin, but their occurrence in fetal skin is unknown. T cell and Langerhans cell (LC) populations were studied using single or double immunohistochemical staining on cryostat-section. Skin samples taken from different body regions of 17 fetuses ranging from 18 to 30 weeks estimated gestational-age (w-EGA), were examined. In all specimens but one, we did not find any epidermal T cell. In contrast, dermal CD3(+) T cells occurred at all w-EGA. The density of these cells increased with increasing age. Double staining showed that CD3(+) T cells were predominantly CD4(+)/CD45RA(+). On the other hand, LC, as assessed by CD1a expression, was evenly distributed within the interfollicular epidermis and papillary dermis at all gestational ages. Analysis of T cell and LC density in different body regions did not show significant topographic differences. We suggest that lack of epidermal T cells, although the LC network was fully represented, might reflect the scarce opportunity of fetal LC to contact foreign antigens in utero.
Densities, distribution and phenotypic expression of T cells in human fetal skin / DI NUZZO, Sergio; Pavanello, P; Masotti, A; Giordano, Giovanna; DE PANFILIS, Giuseppe. - In: ARCHIVES OF DERMATOLOGICAL RESEARCH. - ISSN 0340-3696. - 301:10(2009), pp. 753-755. [10.1007/s00403-009-0943-9]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2293894
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