Tryptophan synthase is a pyridoxal 5'-phosphate-dependent alpha(2)beta(2) complex catalyzing the last two steps of tryptophan biosynthesis in bacteria, plants and fungi. Structural, dynamic and functional studies, carried out over more than 40 years, have unveiled that: (1) alpha- and beta-active sites are separated by about 20 A and communicate via the selective stabilization of distinct conformational states, triggered by the chemical nature of individual catalytic intermediates and by allosteric ligands; (2) indole, formed at alpha-active site, is intramolecularly channeled to the beta-active site; and (3) naturally occurring as well as genetically generated mutants have allowed to pinpoint functional and regulatory roles for several individual amino acids. These key features have made tryptophan synthase a text-book case for the understanding of the interplay between chemistry and conformational energy landscapes.

Tryptophan synthase: a mine for enzymologists / Raboni, Samanta; Bettati, Stefano; Mozzarelli, Andrea. - In: CELLULAR AND MOLECULAR LIFE SCIENCES. - ISSN 1420-682X. - 66:(2009), pp. 2391-2403. [10.1007/s00018-009-0028-0]

Tryptophan synthase: a mine for enzymologists

RABONI, Samanta;BETTATI, Stefano;MOZZARELLI, Andrea
2009-01-01

Abstract

Tryptophan synthase is a pyridoxal 5'-phosphate-dependent alpha(2)beta(2) complex catalyzing the last two steps of tryptophan biosynthesis in bacteria, plants and fungi. Structural, dynamic and functional studies, carried out over more than 40 years, have unveiled that: (1) alpha- and beta-active sites are separated by about 20 A and communicate via the selective stabilization of distinct conformational states, triggered by the chemical nature of individual catalytic intermediates and by allosteric ligands; (2) indole, formed at alpha-active site, is intramolecularly channeled to the beta-active site; and (3) naturally occurring as well as genetically generated mutants have allowed to pinpoint functional and regulatory roles for several individual amino acids. These key features have made tryptophan synthase a text-book case for the understanding of the interplay between chemistry and conformational energy landscapes.
Tryptophan synthase: a mine for enzymologists / Raboni, Samanta; Bettati, Stefano; Mozzarelli, Andrea. - In: CELLULAR AND MOLECULAR LIFE SCIENCES. - ISSN 1420-682X. - 66:(2009), pp. 2391-2403. [10.1007/s00018-009-0028-0]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2293687
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