Abstract: In a previous study, an apathogenic strain of bovine herpesvirus 4 (BoHV-4) cloned as a bacterial artificial chromosome and expressing a chimeric peptide (gE2/gD) as a secreted form was described. Recombinant virus-inoculated animals produced antibodies against bovine viral diarrhea virus (BVDV) gE2 and BoHV-1 gD. However, neutralizing antibodies were produced only against BVDV, not against BoHV-1. In the present work a recombinant BoHV-4 expressing a membrane-linked form of gE2/gD chimeric peptide was constructed, and inoculated rabbits produced serum-neutralizing antibodies against both BVDV and BoHV-1. Protein cell sorting and targeting are a very important issue when immunodominant antigens are engineered for recombinant virus vaccine development
Cellular targeting of engineered heterologous antigens is a determinant factor for bovine herpesvirus 4- based vaccine vector development / Donofrio, Gaetano; Franceschi, Valentina; Capocefalo, Antonio; Taddei, Simone; Sartori, Chiara; Bonomini, S; Cavirani, Sandro; Cabassi, Clotilde Silvia; Flammini, Cesidio Filippo. - In: CLINICAL AND VACCINE IMMUNOLOGY. - ISSN 1556-6811. - 16:11(2009), pp. 1675-1686. [10.1128/CVI.00224-09]
Cellular targeting of engineered heterologous antigens is a determinant factor for bovine herpesvirus 4- based vaccine vector development
DONOFRIO, Gaetano;FRANCESCHI, Valentina;CAPOCEFALO, Antonio;TADDEI, Simone;SARTORI, Chiara;CAVIRANI, Sandro;CABASSI, Clotilde Silvia;FLAMMINI, Cesidio Filippo
2009-01-01
Abstract
Abstract: In a previous study, an apathogenic strain of bovine herpesvirus 4 (BoHV-4) cloned as a bacterial artificial chromosome and expressing a chimeric peptide (gE2/gD) as a secreted form was described. Recombinant virus-inoculated animals produced antibodies against bovine viral diarrhea virus (BVDV) gE2 and BoHV-1 gD. However, neutralizing antibodies were produced only against BVDV, not against BoHV-1. In the present work a recombinant BoHV-4 expressing a membrane-linked form of gE2/gD chimeric peptide was constructed, and inoculated rabbits produced serum-neutralizing antibodies against both BVDV and BoHV-1. Protein cell sorting and targeting are a very important issue when immunodominant antigens are engineered for recombinant virus vaccine development| File | Dimensione | Formato | |
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