Complex of 2-thiophenecarbonyl and isonicotinoyl hydrazones of 3-(N-methyl)isatin. A study of their antimicrobial activity

Cobalt(II), nickel(II), copper(II) and zinc(II) complexes of 2-thiophenecarbonyl and isonicotinoyl hydrazones of 3-(N-methyl)isatin (HL1 and HL2, respectively) were synthesized and characterized, being the crystal structures of HL1, HL2 and [Ni(L1)2] · 2CHCl3 elucidated by X-ray diffraction techniques. The in vitro antimicrobial activity of all these compounds was tested against several bacteria and fungi. HL1and its complexes exhibited a strong inhibition of the growth of Haemophilus influenzae (MIC 0.15–1.50 μg/mL) and good antibacterial properties towards Bacillus subtilis (MIC 3–25 μg/mL). The minimal inhibitory concentration (MIC) was defined as the lowest concentration of compound inhibiting the growth of each strain. The antibacterial effectiveness was confirmed against a number of Gram positive bacteria, including methicillin-resistant Staphylococcus aureus. Yeasts and moulds showed a low susceptibility, except the dermatophyte mould Epidermophyton floccosum that is inhibited at concentrations ranging from 6 to 50 μg/mL. In general, the antimicrobial activity of the thiophene derivatives was greater than that of the isonicotinic analogues.