CD56(bright) natural killer (NK) cells, generated in vitro from CD34+ hematopoietic progenitor cells, were characterized after a 30-day culture with flt3 ligand plus IL-15. Virtually, all CD56(bright) cells expressed CD117, CD25, natural cytotoxicity receptors (NCRs), NKG2D, CD161, and CD244, while only a subset expressed CD18-CD11a (LFA-1), and CD94 molecule, defining an immature CD56(bright)/NCRs+/NKG2D+/LFA-1(-)/CD94(-) subset. Another small subset of cells expressing CD94 but not LFA-1 integrin was also identified, suggesting that during NK differentiation LFA-1 might be upregulated later than CD94. To verify this hypothesis in vivo, we evaluated the NK cell expression of LFA-1 in both peripheral and umbilical cord blood samples. Interestingly, in these blood fluids, we have identified a lineage negative CD34(-)/LFA-1(low)/NKp46(dim)/NKG2D(dim)/CD94(-) subset that resembled an immature stage of NK cells present in lymph nodes. Altogether, the results indicate that CD18-CD11a integrin, as well as CD11b in mice, may be a useful marker to identify immature stages of NK cell differentiation.

Identification of a NCR+/NKG2D+/LFA-1(low)/CD94(-) immature human NK cell subset / ZAMAI L.; GALEOTTI L.; ZOTTO G.D.; CANONICO B.; MIRANDOLA P.; PAPA S.. - In: CYTOMETRY. PART A. - ISSN 1552-4922. - 75(2009), pp. 893-901. [10.1002/cyto.a.20789]

Identification of a NCR+/NKG2D+/LFA-1(low)/CD94(-) immature human NK cell subset.

MIRANDOLA, Prisco;
2009

Abstract

CD56(bright) natural killer (NK) cells, generated in vitro from CD34+ hematopoietic progenitor cells, were characterized after a 30-day culture with flt3 ligand plus IL-15. Virtually, all CD56(bright) cells expressed CD117, CD25, natural cytotoxicity receptors (NCRs), NKG2D, CD161, and CD244, while only a subset expressed CD18-CD11a (LFA-1), and CD94 molecule, defining an immature CD56(bright)/NCRs+/NKG2D+/LFA-1(-)/CD94(-) subset. Another small subset of cells expressing CD94 but not LFA-1 integrin was also identified, suggesting that during NK differentiation LFA-1 might be upregulated later than CD94. To verify this hypothesis in vivo, we evaluated the NK cell expression of LFA-1 in both peripheral and umbilical cord blood samples. Interestingly, in these blood fluids, we have identified a lineage negative CD34(-)/LFA-1(low)/NKp46(dim)/NKG2D(dim)/CD94(-) subset that resembled an immature stage of NK cells present in lymph nodes. Altogether, the results indicate that CD18-CD11a integrin, as well as CD11b in mice, may be a useful marker to identify immature stages of NK cell differentiation.
Identification of a NCR+/NKG2D+/LFA-1(low)/CD94(-) immature human NK cell subset / ZAMAI L.; GALEOTTI L.; ZOTTO G.D.; CANONICO B.; MIRANDOLA P.; PAPA S.. - In: CYTOMETRY. PART A. - ISSN 1552-4922. - 75(2009), pp. 893-901. [10.1002/cyto.a.20789]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2288282
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