Silymarin has been used as a supplement in chicken diet to prevent aflatoxicosis. Compelling evidence indicate that flavonoids are endocrine disruptors. Therefore, this study was aimed to evaluate the effects of silymarin on steroidogenesis in hen follicular wall explants. Eighteen laying hens were randomly allotted into 3 treatment groups; control hens were fed with basal diet, while a supplementation with 200- 400 ppm of silymarin was carried out in group S200 and S400. After 5 months, hens were sacrificed and hierarchycal follicles, classified according to their increasing size from F5/6 to F1, were removed, slit and carefully inverted. Individual follicles were placed into a single well of a 6-well plate and incubated for 48h. At the end, media were collected and assayed for progesterone (P4) and estradiol-17 beta (E2) content by RIA. Basal E2 production significantly increased (P<0.05) from F5 to F3, then significantly decreased (p<0.05) from F3 to F1. Basal P4 production significantly augmented (p<0.05) throughout all follicle growth. Both silymarin concentrations were able to significanlty inhibit (p<0.05) steroidogenesis. Taken together our results suggest that silymarin can effectively act as an endocrine-disrupting chemical: the potential impact of a diet silymarin supplementation on animal health and productivity should be addressed.
The flavonoid Silymarin inhibits steroidogenesis in cultured hen follicle / Basini, Giuseppina; Righi, Federico; Bussolati, Simona; Santini, S. E.; Quarantelli, Afro; Grasselli, Francesca. - In: REPRODUCTION IN DOMESTIC ANIMALS. - ISSN 0936-6768. - 42:(2007), pp. 128-128. (Intervento presentato al convegno ESDAR tenutosi a Celle, Germany nel 19-23 September).
The flavonoid Silymarin inhibits steroidogenesis in cultured hen follicle
BASINI, Giuseppina;RIGHI, Federico;BUSSOLATI, Simona;QUARANTELLI, Afro;GRASSELLI, Francesca
2007-01-01
Abstract
Silymarin has been used as a supplement in chicken diet to prevent aflatoxicosis. Compelling evidence indicate that flavonoids are endocrine disruptors. Therefore, this study was aimed to evaluate the effects of silymarin on steroidogenesis in hen follicular wall explants. Eighteen laying hens were randomly allotted into 3 treatment groups; control hens were fed with basal diet, while a supplementation with 200- 400 ppm of silymarin was carried out in group S200 and S400. After 5 months, hens were sacrificed and hierarchycal follicles, classified according to their increasing size from F5/6 to F1, were removed, slit and carefully inverted. Individual follicles were placed into a single well of a 6-well plate and incubated for 48h. At the end, media were collected and assayed for progesterone (P4) and estradiol-17 beta (E2) content by RIA. Basal E2 production significantly increased (P<0.05) from F5 to F3, then significantly decreased (p<0.05) from F3 to F1. Basal P4 production significantly augmented (p<0.05) throughout all follicle growth. Both silymarin concentrations were able to significanlty inhibit (p<0.05) steroidogenesis. Taken together our results suggest that silymarin can effectively act as an endocrine-disrupting chemical: the potential impact of a diet silymarin supplementation on animal health and productivity should be addressed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
