We report five new complete mitochondrial DNA (mtDNA) ge- nomes of Siberian woolly mammoth (Mammuthus primigenius), sequenced with up to 73-fold coverage from DNA extracted from hair shaft material. Three of the sequences present the first complete mtDNA genomes of mammoth clade II. Analysis of these and 13 recently published mtDNA genomes demonstrates the existence of two apparently sympatric mtDNA clades that exhibit high interclade divergence. The analytical power afforded by the analysis of the complete mtDNA genomes reveals a surprisingly ancient coalescence age of the two clades, 1–2 million years, depending on the calibration technique. Furthermore, statistical analysis of the temporal distribution of the 14C ages of these and previously identified members of the two mammoth clades sug- gests that clade II went extinct before clade I. Modeling of protein structures failed to indicate any important functional difference between genomes belonging to the two clades, suggesting that the loss of clade IImore likely is due to genetic drift than a selective sweep.
Intraspecific phylogenetic analysis of siberian woolly mammoths using complete mitochondrial genomes / Gilbert, M. T. P.; L. P., Tomsho; S., Rendulic; M., Packard; D. I., Drautz; A., Sher; A. I., Tikhonov; Ji, Qi; I., Barnes; L., D.; B., Shapiro; CHIH HAO, Hsu; A., Lister; T., Kuznetsova; P., Kosintsev; P. F., Campos; J., Humphrey; T. S., Higham; A. S., Wilson; B., Buigues; PER G. P., Ericson; M., Germonpré; A., Götherström; Iacumin, Paola; V., Nikolaev; M., NOWAK KEMP; E., Willerslev; A. M., Lesk; J. R., Knight; G, ; P., Irzyk; C. S., Perbost; K. M., Fredrikson; T. T., Harkins; S., Sheridan; W. MILLER AND S. C., Schuster. - In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. - ISSN 1091-6490. - 105:(2008), pp. 8327-8332.
Intraspecific phylogenetic analysis of siberian woolly mammoths using complete mitochondrial genomes
IACUMIN, Paola;
2008-01-01
Abstract
We report five new complete mitochondrial DNA (mtDNA) ge- nomes of Siberian woolly mammoth (Mammuthus primigenius), sequenced with up to 73-fold coverage from DNA extracted from hair shaft material. Three of the sequences present the first complete mtDNA genomes of mammoth clade II. Analysis of these and 13 recently published mtDNA genomes demonstrates the existence of two apparently sympatric mtDNA clades that exhibit high interclade divergence. The analytical power afforded by the analysis of the complete mtDNA genomes reveals a surprisingly ancient coalescence age of the two clades, 1–2 million years, depending on the calibration technique. Furthermore, statistical analysis of the temporal distribution of the 14C ages of these and previously identified members of the two mammoth clades sug- gests that clade II went extinct before clade I. Modeling of protein structures failed to indicate any important functional difference between genomes belonging to the two clades, suggesting that the loss of clade IImore likely is due to genetic drift than a selective sweep.| File | Dimensione | Formato | |
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