Abstract: Bovine herpesvirus 4 (BoHV-4) is a gamma-herpesvirus with no clear disease association. A recombinant BoHV-4 (BoHV-4EGFPΔTK) expressing Green Fluorescent Protein (EGFP), was successfully used to infect F98 rat glioma cells. BoHV-4EGFPΔTK was injected into the lateral ventricle of the rat brain. Histology and immunohistochemistry showed that ependymal and rostral migratory stream cells were transduced while neurons were not. Clinical scores, evaluated for 90 days, indicated that the virus was non neuropathogenic, suggesting this virus is a suitable vector for brain tumor gene therapy.
Transduction of the rat brain by Bovine Herpesvirus 4 / Redaelli, M; Cavaggioni, A; MUCIGNAT CARETTA, C; Cavirani, Sandro; Caretta, Antonio; Donofrio, Gaetano. - In: GENETIC VACCINES AND THERAPY. - ISSN 1479-0556. - (2008), pp. 1-4. [10.1186/1479-0556-6-6]
Transduction of the rat brain by Bovine Herpesvirus 4.
CAVIRANI, Sandro;CARETTA, Antonio;DONOFRIO, Gaetano
2008-01-01
Abstract
Abstract: Bovine herpesvirus 4 (BoHV-4) is a gamma-herpesvirus with no clear disease association. A recombinant BoHV-4 (BoHV-4EGFPΔTK) expressing Green Fluorescent Protein (EGFP), was successfully used to infect F98 rat glioma cells. BoHV-4EGFPΔTK was injected into the lateral ventricle of the rat brain. Histology and immunohistochemistry showed that ependymal and rostral migratory stream cells were transduced while neurons were not. Clinical scores, evaluated for 90 days, indicated that the virus was non neuropathogenic, suggesting this virus is a suitable vector for brain tumor gene therapy.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
