The paper describes two methods that allow rapid and accurate identification of Lactobacillus helveticus strains based on the nucleotide sequence of the gene coding for the surface layer (S-layer) protein. The first method is based on a polymerase chain reaction amplification using primers targeting a specific fragment of the S-layer gene. The second method involved a portion of the S-layer gene of L. helveticus as a probe in a hybridisation test. The specificity of these protocols was checked for DNA samples isolated from various Lactobacillus strains

S-layer gene as a molecular marker for identification of Lactobacillus helveticus / Ventura, Marco; L. MORELLI AND M. L., Callegari. - In: FEMS MICROBIOLOGY LETTERS. - ISSN 0378-1097. - 289:(2000), pp. 275-279.

S-layer gene as a molecular marker for identification of Lactobacillus helveticus

VENTURA, Marco;
2000-01-01

Abstract

The paper describes two methods that allow rapid and accurate identification of Lactobacillus helveticus strains based on the nucleotide sequence of the gene coding for the surface layer (S-layer) protein. The first method is based on a polymerase chain reaction amplification using primers targeting a specific fragment of the S-layer gene. The second method involved a portion of the S-layer gene of L. helveticus as a probe in a hybridisation test. The specificity of these protocols was checked for DNA samples isolated from various Lactobacillus strains
2000
S-layer gene as a molecular marker for identification of Lactobacillus helveticus / Ventura, Marco; L. MORELLI AND M. L., Callegari. - In: FEMS MICROBIOLOGY LETTERS. - ISSN 0378-1097. - 289:(2000), pp. 275-279.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/1721286
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