A transcriptional analysis of the lysogeny-related genes of the temperate bacteriophage Lactococcus lactis phiLC3 was performed using Northern blot hybridization during lysogeny and lytic infection by the phage. The lysogeny-related gene cluster was found to contain four promoters (P(1), P(2), Pint and P(173)), while the P(87) promoter directed transcription of orf80 and the putative gene orf87, which are located between the integrase gene and the cell lysis genes. The start sites of the transcripts were determined by primer extension. The divergently oriented lysogenic P(1) and lytic P(2) promoters located in the genetic switch region are responsible for transcription of orf286 which encodes the phage repressor, and the genes orf63 - orf76 - orf236 - orf110 - orf82 - orf57, respectively, while orf173 is transcribed from P(173). orf76 was identified as the gene encoding the Cro-like protein of phiLC3, and it was shown that ORF76 is able to bind specifically to the genetic switch region, albeit with lower affinity than does the phage repressor ORF286. ORF76 also competed with ORF286 for binding to this region. The functionality of P(1) and P(2), and their regulation by ORF286 and ORF76, was investigated using a reporter gene. In general, P(2) was a stronger promoter than P(1), but expression from both promoters, especially P(2), was regulated and modulated by flanking sequences and the presence of orf286 and orf76. ORF286 and ORF76 were both able to repress transcription from P(1) and P(2), while ORF286 was able to stimulate its own synthesis by tenfold. This work reveals the complex interplay between the regulatory elements that control the genetic switch between lysis and lysogeny in phiLC3 and other temperate phages of Lactococcus.

Transcriptional and regulatory studies of the genetic elements involved in the lysogenic-lytic commitment and the identification of the cro-like protein ORF76 of the temperate lactococcal bacteriophage ΦLC3 / Blatny, J. M.; Ventura, Marco; E. M., Rosenhaven; P. A., Risoen; M., Lunde; H., Brssow; AND I. F., Nes. - In: MOLECULAR GENETICS AND GENOMICS. - ISSN 1617-4615. - 269:(2003), pp. 487-498. [10.1007/s00438-003-0854-y]

Transcriptional and regulatory studies of the genetic elements involved in the lysogenic-lytic commitment and the identification of the cro-like protein ORF76 of the temperate lactococcal bacteriophage ΦLC3

VENTURA, Marco;
2003-01-01

Abstract

A transcriptional analysis of the lysogeny-related genes of the temperate bacteriophage Lactococcus lactis phiLC3 was performed using Northern blot hybridization during lysogeny and lytic infection by the phage. The lysogeny-related gene cluster was found to contain four promoters (P(1), P(2), Pint and P(173)), while the P(87) promoter directed transcription of orf80 and the putative gene orf87, which are located between the integrase gene and the cell lysis genes. The start sites of the transcripts were determined by primer extension. The divergently oriented lysogenic P(1) and lytic P(2) promoters located in the genetic switch region are responsible for transcription of orf286 which encodes the phage repressor, and the genes orf63 - orf76 - orf236 - orf110 - orf82 - orf57, respectively, while orf173 is transcribed from P(173). orf76 was identified as the gene encoding the Cro-like protein of phiLC3, and it was shown that ORF76 is able to bind specifically to the genetic switch region, albeit with lower affinity than does the phage repressor ORF286. ORF76 also competed with ORF286 for binding to this region. The functionality of P(1) and P(2), and their regulation by ORF286 and ORF76, was investigated using a reporter gene. In general, P(2) was a stronger promoter than P(1), but expression from both promoters, especially P(2), was regulated and modulated by flanking sequences and the presence of orf286 and orf76. ORF286 and ORF76 were both able to repress transcription from P(1) and P(2), while ORF286 was able to stimulate its own synthesis by tenfold. This work reveals the complex interplay between the regulatory elements that control the genetic switch between lysis and lysogeny in phiLC3 and other temperate phages of Lactococcus.
2003
Transcriptional and regulatory studies of the genetic elements involved in the lysogenic-lytic commitment and the identification of the cro-like protein ORF76 of the temperate lactococcal bacteriophage ΦLC3 / Blatny, J. M.; Ventura, Marco; E. M., Rosenhaven; P. A., Risoen; M., Lunde; H., Brssow; AND I. F., Nes. - In: MOLECULAR GENETICS AND GENOMICS. - ISSN 1617-4615. - 269:(2003), pp. 487-498. [10.1007/s00438-003-0854-y]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/1721133
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