Purpose: The X and Ychromosomes have been associated with malignancy in different types of human tumors. This study attempts to determine the involvement of X chromosome and pseucloautosomal regions (PAR) in sporadic colorectal carcinogenesis. Experimental Design: An allelotyping of X chromosome in 20 premalignant and 22 malignant sporadic colorectal tumors (CRC) from female patients and an analysis of losses [loss of heterozygosity (LOH)] on PARs from 44 CRCs and 12 adenomas of male patients were carried out. In male tumors, a fluorescence in situ hybridization analysis was done to identify which sex chromosome was possibly lost. Results: The LOH frequency in female CRCs was 46% with higher incidence in patients with tumor recurrence than in those who were disease-free (P < 0.01) and with a significant difference from adenomas (11%; P < 0.0001).The LOH rate of PARs in male CRCs was 37% with a frequency significantly higher in patients with recurrence (P < 0.03). These results were maintained also when data from PARs of all 66 male and female patients were cumulated (P < 0.05). LOH in FARs was significantly correlated with LOH at 5q (P < 0.01) and 18q (P < 0.01), early and late events, respectively, in colorectal carcinogenesis. Fluorescence in situ hybridization analysis in male patients with extensive PAR LOH revealed a preferential loss of the Y chromosome. Conclusions: Our data suggest a role for sex chromosome deletions in the malignant progression of sporadic CRCs and support the presence in the PARs of putative tumor suppressor genes involved in the progression of human sporadic CRCs.

Sex chromosome alterations associate with tumor progression in sporadic colorectal carcinomas / Bottarelli, L; Azzoni, C; Necchi, F; Lagrasta, Costanza Anna Maria; Tamburini, E; D'Adda, Tiziana; Pizzi, S; Sarli, Leopoldo; Rindi, G; Bordi, C.. - In: CLINICAL CANCER RESEARCH. - ISSN 1078-0432. - 13:(2007), pp. 4365-4370. [10.1158/1078-0432.CCR-06-2736]

Sex chromosome alterations associate with tumor progression in sporadic colorectal carcinomas.

LAGRASTA, Costanza Anna Maria;D'ADDA, Tiziana;PIZZI S;SARLI, Leopoldo;
2007-01-01

Abstract

Purpose: The X and Ychromosomes have been associated with malignancy in different types of human tumors. This study attempts to determine the involvement of X chromosome and pseucloautosomal regions (PAR) in sporadic colorectal carcinogenesis. Experimental Design: An allelotyping of X chromosome in 20 premalignant and 22 malignant sporadic colorectal tumors (CRC) from female patients and an analysis of losses [loss of heterozygosity (LOH)] on PARs from 44 CRCs and 12 adenomas of male patients were carried out. In male tumors, a fluorescence in situ hybridization analysis was done to identify which sex chromosome was possibly lost. Results: The LOH frequency in female CRCs was 46% with higher incidence in patients with tumor recurrence than in those who were disease-free (P < 0.01) and with a significant difference from adenomas (11%; P < 0.0001).The LOH rate of PARs in male CRCs was 37% with a frequency significantly higher in patients with recurrence (P < 0.03). These results were maintained also when data from PARs of all 66 male and female patients were cumulated (P < 0.05). LOH in FARs was significantly correlated with LOH at 5q (P < 0.01) and 18q (P < 0.01), early and late events, respectively, in colorectal carcinogenesis. Fluorescence in situ hybridization analysis in male patients with extensive PAR LOH revealed a preferential loss of the Y chromosome. Conclusions: Our data suggest a role for sex chromosome deletions in the malignant progression of sporadic CRCs and support the presence in the PARs of putative tumor suppressor genes involved in the progression of human sporadic CRCs.
2007
Sex chromosome alterations associate with tumor progression in sporadic colorectal carcinomas / Bottarelli, L; Azzoni, C; Necchi, F; Lagrasta, Costanza Anna Maria; Tamburini, E; D'Adda, Tiziana; Pizzi, S; Sarli, Leopoldo; Rindi, G; Bordi, C.. - In: CLINICAL CANCER RESEARCH. - ISSN 1078-0432. - 13:(2007), pp. 4365-4370. [10.1158/1078-0432.CCR-06-2736]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/1640339
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