We show that the extent of stable DNA wrapping by Escherichia coli RNA polymerase (RNAP) in the RNAP-promoter open complex depends on the sequence of the promoter and, in particular, on the sequence of the upstream region of the promoter. We further show that the extent of stable DNA wrapping depends on the presence of the RNAP alpha-subunit carboxy-terminal domain and on the presence and length of the RNAP alpha-subunit interdomain linker. Our results indicate that the extensive stable DNA wrapping observed previously in the RNAP-promoter open complex at the lambda P(R) promoter is not a general feature of RNAP-promoter open complexes.

Upstream promoter sequences and alphaCTD mediate stable DNA wrapping within the RNA polymerase-promoter open complex / CELLAI S; MANGIAROTTI L; VANNINI N; NARYSHKIN N; KORTKHONJIA E; EBRIGHT R.H; RIVETTI C.. - In: EMBO REPORTS. - ISSN 1469-221X. - 8(2007), pp. 271-278. [10.1038/sj.embor.7400888]

Upstream promoter sequences and alphaCTD mediate stable DNA wrapping within the RNA polymerase-promoter open complex

CELLAI, Sara;RIVETTI, Claudio
2007

Abstract

We show that the extent of stable DNA wrapping by Escherichia coli RNA polymerase (RNAP) in the RNAP-promoter open complex depends on the sequence of the promoter and, in particular, on the sequence of the upstream region of the promoter. We further show that the extent of stable DNA wrapping depends on the presence of the RNAP alpha-subunit carboxy-terminal domain and on the presence and length of the RNAP alpha-subunit interdomain linker. Our results indicate that the extensive stable DNA wrapping observed previously in the RNAP-promoter open complex at the lambda P(R) promoter is not a general feature of RNAP-promoter open complexes.
Upstream promoter sequences and alphaCTD mediate stable DNA wrapping within the RNA polymerase-promoter open complex / CELLAI S; MANGIAROTTI L; VANNINI N; NARYSHKIN N; KORTKHONJIA E; EBRIGHT R.H; RIVETTI C.. - In: EMBO REPORTS. - ISSN 1469-221X. - 8(2007), pp. 271-278. [10.1038/sj.embor.7400888]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/1631060
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