A novel ICP-MS-based ELISA immunoassay via element-tagged determination was devised for quantitative analysis of hidden allergens in food. The method was able to detect low amounts of peanuts (down to approximately 2 mg peanuts kg−1 cereal-based matrix) by using a europium-tagged antibody. Selectivity was proved by the lack of detectable cross-reaction with a number of protein-rich raw materials.
ICP-MS as a novel detection system for quantitative element-tagged immunoassay of hidden peanut allergens in foods / Careri, Maria; Elviri, Lisa; Mangia, Alessandro; Mucchino, Claudio. - In: ANALYTICAL AND BIOANALYTICAL CHEMISTRY. - ISSN 1618-2642. - 387:(2007), pp. 1851-1854. [10.1007/s00216-006-1091-0]
ICP-MS as a novel detection system for quantitative element-tagged immunoassay of hidden peanut allergens in foods.
CARERI, Maria;ELVIRI, Lisa;MANGIA, Alessandro;MUCCHINO, Claudio
2007-01-01
Abstract
A novel ICP-MS-based ELISA immunoassay via element-tagged determination was devised for quantitative analysis of hidden allergens in food. The method was able to detect low amounts of peanuts (down to approximately 2 mg peanuts kg−1 cereal-based matrix) by using a europium-tagged antibody. Selectivity was proved by the lack of detectable cross-reaction with a number of protein-rich raw materials.| File | Dimensione | Formato | |
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