Hydrogen sulfide, together with carbon monoxide and nitric oxide, is now considered a gasotransmitter able to induce specific cellular responses. As hydrogen sulfide is a component of several natural compounds known to be effective in many inflammatory pathologies, particularly of the respiratory tract, we studied its effects in vitro on the survival and bactericidal activity of purified human neutrophils. We found that (1) HS(-) ions promote the survival of granulocytes, but not that of lymphocytes or eosinophils, cultured in serum-free medium; (2) the pro-survival effect of HS(-) is due to inhibition of caspase-3 cleavage and p38 MAP kinase phosphorylation; (3) the bactericidal activity of neutrophils is not impaired by hydrogen sulfide. We conclude that HS(-) promotes the short-term survival of neutrophils potentially accelerating the resolution of inflammatory processes and preventing the occurrence of new ones.

Hydrogen sulfide prevents apoptosis of human PMN via inhibition of p38 and caspase 3 / Rinaldi, Laura; Gobbi, Giuliana; Pambianco, M.; Micheloni, Cristina; Mirandola, Prisco; Vitale, Marco. - In: LABORATORY INVESTIGATION. - ISSN 0023-6837. - 86(4):(2006), pp. 391-397. [10.1038/labinvest.3700391]

Hydrogen sulfide prevents apoptosis of human PMN via inhibition of p38 and caspase 3

RINALDI, Laura;GOBBI, Giuliana;MICHELONI, Cristina;MIRANDOLA, Prisco;VITALE, Marco
2006-01-01

Abstract

Hydrogen sulfide, together with carbon monoxide and nitric oxide, is now considered a gasotransmitter able to induce specific cellular responses. As hydrogen sulfide is a component of several natural compounds known to be effective in many inflammatory pathologies, particularly of the respiratory tract, we studied its effects in vitro on the survival and bactericidal activity of purified human neutrophils. We found that (1) HS(-) ions promote the survival of granulocytes, but not that of lymphocytes or eosinophils, cultured in serum-free medium; (2) the pro-survival effect of HS(-) is due to inhibition of caspase-3 cleavage and p38 MAP kinase phosphorylation; (3) the bactericidal activity of neutrophils is not impaired by hydrogen sulfide. We conclude that HS(-) promotes the short-term survival of neutrophils potentially accelerating the resolution of inflammatory processes and preventing the occurrence of new ones.
2006
Hydrogen sulfide prevents apoptosis of human PMN via inhibition of p38 and caspase 3 / Rinaldi, Laura; Gobbi, Giuliana; Pambianco, M.; Micheloni, Cristina; Mirandola, Prisco; Vitale, Marco. - In: LABORATORY INVESTIGATION. - ISSN 0023-6837. - 86(4):(2006), pp. 391-397. [10.1038/labinvest.3700391]
File in questo prodotto:
File Dimensione Formato  
LabInvest2006.pdf

non disponibili

Tipologia: Documento in Post-print
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 336.96 kB
Formato Adobe PDF
336.96 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/1502327
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 132
  • ???jsp.display-item.citation.isi??? 126
social impact