Differential display was used to isolate cDNA clones showing differential expression in response to ABA, drought and cold in barley seedling shoots. One drought-regulated cDNA clone (DD12) was further analyzed and found to encode a branched-chain amino acid aminotransferase (HvBCAT-1). A genomic clone was isolated by probing the Morex BAC library with the cDNA clone DD12 and the structure of Hvbcat-1 was elucidated. The coding region is interrupted by six introns and contains a predicted mitochondrial transit peptide. Hvbcat1 was mapped to chromosome 4H. A comparison was made to rice and Arabidopsis genes to identify conserved structural patterns. Complementation of a yeast (Saccharomyces cerevisiae) double knockout strain revealed that HvBCAT-1 can function as the mitochondrial (catabolic) BCATs in vivo. Transcript levels of Hvbcat-1, increased in response to drought stress. As the first enzyme in the branched-chain amino acid (BCAA) catabolic pathway, HvBCAT-1 might have a role in the degradation of BCAA. Degradation of BCAA could serve as a detoxification mechanism that maintains the pool of free branched-chain amino acids at low and non toxic levels, under drought stress conditions.

A BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE GENE ISOLATED FROM HORDEUM VULGARE IS DIFFERENTIALLY REGULATED BY DROUGHT STRESS / MALATRASI M; CORRADI M; SVENSSON I; CLOSE T; GULLI M.; MARMIROLI N. - In: THEORETICAL AND APPLIED GENETICS. - ISSN 0040-5752. - 113(2006), pp. 965-976. [10.1007/s00122-006-0339-6]

A BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE GENE ISOLATED FROM HORDEUM VULGARE IS DIFFERENTIALLY REGULATED BY DROUGHT STRESS

GULLI', Mariolina;MARMIROLI, Nelson
2006

Abstract

Differential display was used to isolate cDNA clones showing differential expression in response to ABA, drought and cold in barley seedling shoots. One drought-regulated cDNA clone (DD12) was further analyzed and found to encode a branched-chain amino acid aminotransferase (HvBCAT-1). A genomic clone was isolated by probing the Morex BAC library with the cDNA clone DD12 and the structure of Hvbcat-1 was elucidated. The coding region is interrupted by six introns and contains a predicted mitochondrial transit peptide. Hvbcat1 was mapped to chromosome 4H. A comparison was made to rice and Arabidopsis genes to identify conserved structural patterns. Complementation of a yeast (Saccharomyces cerevisiae) double knockout strain revealed that HvBCAT-1 can function as the mitochondrial (catabolic) BCATs in vivo. Transcript levels of Hvbcat-1, increased in response to drought stress. As the first enzyme in the branched-chain amino acid (BCAA) catabolic pathway, HvBCAT-1 might have a role in the degradation of BCAA. Degradation of BCAA could serve as a detoxification mechanism that maintains the pool of free branched-chain amino acids at low and non toxic levels, under drought stress conditions.
A BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE GENE ISOLATED FROM HORDEUM VULGARE IS DIFFERENTIALLY REGULATED BY DROUGHT STRESS / MALATRASI M; CORRADI M; SVENSSON I; CLOSE T; GULLI M.; MARMIROLI N. - In: THEORETICAL AND APPLIED GENETICS. - ISSN 0040-5752. - 113(2006), pp. 965-976. [10.1007/s00122-006-0339-6]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/1493284
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