The a-hydroxyacid 2-hydroxy-4-methylthiobutanoic acid (the so-called methionine hydroxy-analogue, MHA), largely used in animal nutrition as a source of methionine, forms stable metal chelates with divalent metals of formula [{CH3SCH2 CH2CH(OH)- COO}2M] Æ nH2O. Protonation and iron(III) and copper(II) complex formation constants have been determined by potentiometry at 25 C. Distribution diagrams show that no free Fe3+ cations are present in solution at pH > 2.5. ESI-MS (Electron- Spray Ionization Mass Spectrometry) investigations carried out both on iron and zinc complexes in solution have evidenced various species with different MHA/metal ratios. In vivo trials were carried out with rats. After receiving a zinc-deficient diet for 3 weeks, animals were fed the same diet added with zinc sulfate or zinc/MHA chelate; the zinc content of faeces was higher (+45%; P < 0.05) in sulfate fed rats, whereas zinc retention was higher (+61%; P < 0.05) in the Zn/MHA diet. Experiments in vitro with human intestinal Caco-2 cells indicated that the MHA/Fe chelate was taken up by the cells without any apparent toxic effect. The iron uptake was higher than that of iron nitrilotriacetate (Fe3+NTA), an effective chelate for delivering iron to milk diets. In conclusion, these data indicate that the use of MHA chelates could be a valuable tool to increase bioavailability of trace minerals and reduce the environmental impact of animal manure.

Metal Chelates of 2-hydroxy-4-methylbutanoic acid in animal feeding. Part 2: further characterization, in vitro and in vivo investigations / Predieri, Giovanni; Elviri, Lisa; Tegoni, Matteo; Zagnoni, I.; Cinti, E.; Biagi, G.; Ferruzza, S.; Leonardi, G.. - In: JOURNAL OF INORGANIC BIOCHEMISTRY. - ISSN 0162-0134. - 99:(2005), pp. 627-636. [10.1016/j.jinorgbio.2004.11.011]

Metal Chelates of 2-hydroxy-4-methylbutanoic acid in animal feeding. Part 2: further characterization, in vitro and in vivo investigations

PREDIERI, Giovanni;ELVIRI, Lisa;TEGONI, Matteo;
2005-01-01

Abstract

The a-hydroxyacid 2-hydroxy-4-methylthiobutanoic acid (the so-called methionine hydroxy-analogue, MHA), largely used in animal nutrition as a source of methionine, forms stable metal chelates with divalent metals of formula [{CH3SCH2 CH2CH(OH)- COO}2M] Æ nH2O. Protonation and iron(III) and copper(II) complex formation constants have been determined by potentiometry at 25 C. Distribution diagrams show that no free Fe3+ cations are present in solution at pH > 2.5. ESI-MS (Electron- Spray Ionization Mass Spectrometry) investigations carried out both on iron and zinc complexes in solution have evidenced various species with different MHA/metal ratios. In vivo trials were carried out with rats. After receiving a zinc-deficient diet for 3 weeks, animals were fed the same diet added with zinc sulfate or zinc/MHA chelate; the zinc content of faeces was higher (+45%; P < 0.05) in sulfate fed rats, whereas zinc retention was higher (+61%; P < 0.05) in the Zn/MHA diet. Experiments in vitro with human intestinal Caco-2 cells indicated that the MHA/Fe chelate was taken up by the cells without any apparent toxic effect. The iron uptake was higher than that of iron nitrilotriacetate (Fe3+NTA), an effective chelate for delivering iron to milk diets. In conclusion, these data indicate that the use of MHA chelates could be a valuable tool to increase bioavailability of trace minerals and reduce the environmental impact of animal manure.
2005
Metal Chelates of 2-hydroxy-4-methylbutanoic acid in animal feeding. Part 2: further characterization, in vitro and in vivo investigations / Predieri, Giovanni; Elviri, Lisa; Tegoni, Matteo; Zagnoni, I.; Cinti, E.; Biagi, G.; Ferruzza, S.; Leonardi, G.. - In: JOURNAL OF INORGANIC BIOCHEMISTRY. - ISSN 0162-0134. - 99:(2005), pp. 627-636. [10.1016/j.jinorgbio.2004.11.011]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/1446309
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