A new, rapid and sensitive method is reported for the multiresidual determination of type A (diacetoxyscirpenol, HT-2 toxin, T-2 toxin) and type B (nivalenol, deoxynivalenol, fusarenon X, 15-O-acetyl-4-deoxynivalenol) trichothecenes in wheat flour samples. Sample extraction was performed with acetonitrile/water mixtures. Mycosep© columns were used for a fast and effective clean-up procedure. The analytes were separated by HPLC with a RP C18 column by means of a gradient elution and detected in an ESI-interfaced single quadrupole mass spectrometer. Type B and type A trichothecenes were monitored in the negative and in the positive ion mode, respectively. The method performance is reported in terms of linearity (r 2 = 0.999), specificity, accuracy (recoveries from 70–120%) and precision (CV% = 5), the LOQs are in the range 10–20 μg/Kg.

A Rapid Multiresidual Determination of of Type A and Type B Trichothecenes in Cereals and Feeds HPLC-by ESI-MS / Biancardi, A.; Gasparini, M.; Dall'Asta, Chiara; Marchelli, Rosangela. - In: FOOD ADDITIVES AND CONTAMINANTS. - ISSN 0265-203X. - 22:(2005), pp. 251-258. [10.1080/02652030500110923]

A Rapid Multiresidual Determination of of Type A and Type B Trichothecenes in Cereals and Feeds HPLC-by ESI-MS

DALL'ASTA, Chiara;MARCHELLI, Rosangela
2005-01-01

Abstract

A new, rapid and sensitive method is reported for the multiresidual determination of type A (diacetoxyscirpenol, HT-2 toxin, T-2 toxin) and type B (nivalenol, deoxynivalenol, fusarenon X, 15-O-acetyl-4-deoxynivalenol) trichothecenes in wheat flour samples. Sample extraction was performed with acetonitrile/water mixtures. Mycosep© columns were used for a fast and effective clean-up procedure. The analytes were separated by HPLC with a RP C18 column by means of a gradient elution and detected in an ESI-interfaced single quadrupole mass spectrometer. Type B and type A trichothecenes were monitored in the negative and in the positive ion mode, respectively. The method performance is reported in terms of linearity (r 2 = 0.999), specificity, accuracy (recoveries from 70–120%) and precision (CV% = 5), the LOQs are in the range 10–20 μg/Kg.
2005
A Rapid Multiresidual Determination of of Type A and Type B Trichothecenes in Cereals and Feeds HPLC-by ESI-MS / Biancardi, A.; Gasparini, M.; Dall'Asta, Chiara; Marchelli, Rosangela. - In: FOOD ADDITIVES AND CONTAMINANTS. - ISSN 0265-203X. - 22:(2005), pp. 251-258. [10.1080/02652030500110923]
File in questo prodotto:
File Dimensione Formato  
4.pdf

non disponibili

Tipologia: Documento in Post-print
Licenza: Creative commons
Dimensione 195.46 kB
Formato Adobe PDF
195.46 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/1444809
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 27
  • ???jsp.display-item.citation.isi??? 26
social impact