Objectives: To evaluate whether an engineered synthetic decapeptide (KP) derived from the sequence of a recombinant anti-idiotypic antibody, that represents the internal image of a Pichia anomala killer toxin, could be fungicidal in vitro and therapeutic in vivo against Paracoccidioides brasiliensis and paracoccidioidomycosis (PCM). Methods: Fungicidal activity of KP was assessed in vitro and in vivo by inhibition of colony forming units and by histological examination, 8 days after infection, of organs from mice intravenously injected with a virulent strain of P. brasiliensis (3 x 106 yeast cells) and intraperitoneally treated with KP (3.3 micrograms/g body weight, three doses), in comparison with control animals equally administered with a scrambled decapeptide (SP). Results: KP but not SP was fungicidal in vitro at 39 ng/multiply-budding yeast cell and less efficiently in its D-isomeric form (0.31micrograms/multiply-budding yeast cell). It was also able to markedly reduce the fungal load in organs (liver, lung, spleen) of infected animals. Conclusions: The therapeutic effect observed opens the way for using the antifungal peptide as an alternative control of PCM in association with conventional antifungal drugs.
Therapeutic activity of a killer peptide against experimental paracoccidioidomycosis / Travassos, L. R.; Silva, L. S.; Rodrigues, E. G.; Conti, Stefania; Salati, A.; Magliani, Valter; Polonelli, Luciano. - In: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. - ISSN 0305-7453. - 54:(2004), pp. 956-958. [10.1093/jac/dkh430]
Therapeutic activity of a killer peptide against experimental paracoccidioidomycosis
CONTI, Stefania;MAGLIANI, Valter;POLONELLI, Luciano
2004-01-01
Abstract
Objectives: To evaluate whether an engineered synthetic decapeptide (KP) derived from the sequence of a recombinant anti-idiotypic antibody, that represents the internal image of a Pichia anomala killer toxin, could be fungicidal in vitro and therapeutic in vivo against Paracoccidioides brasiliensis and paracoccidioidomycosis (PCM). Methods: Fungicidal activity of KP was assessed in vitro and in vivo by inhibition of colony forming units and by histological examination, 8 days after infection, of organs from mice intravenously injected with a virulent strain of P. brasiliensis (3 x 106 yeast cells) and intraperitoneally treated with KP (3.3 micrograms/g body weight, three doses), in comparison with control animals equally administered with a scrambled decapeptide (SP). Results: KP but not SP was fungicidal in vitro at 39 ng/multiply-budding yeast cell and less efficiently in its D-isomeric form (0.31micrograms/multiply-budding yeast cell). It was also able to markedly reduce the fungal load in organs (liver, lung, spleen) of infected animals. Conclusions: The therapeutic effect observed opens the way for using the antifungal peptide as an alternative control of PCM in association with conventional antifungal drugs.File | Dimensione | Formato | |
---|---|---|---|
Abstract J Antimicrobial Chemother 2004.pdf
non disponibili
Tipologia:
Abstract
Licenza:
NON PUBBLICO - Accesso privato/ristretto
Dimensione
51.59 kB
Formato
Adobe PDF
|
51.59 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
J Antimicrobial Chemother 2004.pdf
non disponibili
Tipologia:
Altro materiale allegato
Licenza:
NON PUBBLICO - Accesso privato/ristretto
Dimensione
149.42 kB
Formato
Adobe PDF
|
149.42 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.