Enzyme-labelled amperometric genosensors and immunosensors based on advanced nanomaterials

The present PhD thesis mainly focused on design, development and validation of amperometric genosensors and genoassays based on immobilization of PNA probes on advanced and highly performing micro- and nanomaterials. The application field of such devices concerned the rapid and reliable determination of non-amplified DNA from genetically modified organisms (i.e. transgenic soy). Other issues faced during the PhD program were i) the development of portable IoT devices for acquisition and storage/sharing of data from biosensors intended for determination of clinically relevant biomarkers and ii) the implementation of piezoelectric mass sensors based on the electropolymerization of cavitands on quartz crystals for monitoring of environmentally relevant aromatic compounds in wastewaters.