: Peptide nucleic acids (PNAs) are emerging tools in the construction of nanostructures possessing self-assembly properties similar to those of DNA but featuring enhanced stability and chemical flexibility. Templated-ligation approaches can be exploited to stabilize the formed nanoproducts, with bio-orthogonal methods preferred for compatibility with natural components. This chapter outlines design requirements and protocols for conducting triggerless bio-orthogonal PNA ligation in solution and on glass surfaces, along with an electrophoretic monitoring protocol for assessing the outcome of solution ligation.
Triggerless Bio-Orthogonal Proximity-Induced PNA Ligation Using 2,5-Dioxopentanyl (DOP) Functionality / Manicardi, A.; Madder, A.. - 2901:(2025), pp. 227-239. [10.1007/978-1-0716-4394-5_17]
Triggerless Bio-Orthogonal Proximity-Induced PNA Ligation Using 2,5-Dioxopentanyl (DOP) Functionality
Manicardi A.
;
2025-01-01
Abstract
: Peptide nucleic acids (PNAs) are emerging tools in the construction of nanostructures possessing self-assembly properties similar to those of DNA but featuring enhanced stability and chemical flexibility. Templated-ligation approaches can be exploited to stabilize the formed nanoproducts, with bio-orthogonal methods preferred for compatibility with natural components. This chapter outlines design requirements and protocols for conducting triggerless bio-orthogonal PNA ligation in solution and on glass surfaces, along with an electrophoretic monitoring protocol for assessing the outcome of solution ligation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
