Peptide Nucleic Acids (PNAs) are oligonucleotide mimics that can be used to block the biological action of microRNA, thus affecting gene expression post-transcriptionally. PNAs are obtained with solid-phase peptide synthesis, and can be easily conjugated to other peptides. Conjugation with R8-Peptide or modification of the PNA backbone (at C5 or C2 carbon) with arginine side chains allows efficient cellular uptake. The present protocol describes the synthesis of cationic PNAs that can be used alone as drugs or for efficient co-delivery in suitable inorganic nanocarriers.

Preparation of anti-miR PNAs for drug development and nanomedicine / Manicardi, Alex; Gambari, Roberto; de Cola, Luisa; Corradini, Roberto. - STAMPA. - 1811:(2018), pp. 49-63. [10.1007/978-1-4939-8582-1_4]

Preparation of anti-miR PNAs for drug development and nanomedicine

Manicardi, Alex;de Cola, Luisa;Corradini, Roberto
2018

Abstract

Peptide Nucleic Acids (PNAs) are oligonucleotide mimics that can be used to block the biological action of microRNA, thus affecting gene expression post-transcriptionally. PNAs are obtained with solid-phase peptide synthesis, and can be easily conjugated to other peptides. Conjugation with R8-Peptide or modification of the PNA backbone (at C5 or C2 carbon) with arginine side chains allows efficient cellular uptake. The present protocol describes the synthesis of cationic PNAs that can be used alone as drugs or for efficient co-delivery in suitable inorganic nanocarriers.
978-1-4939-8581-4
978-1-4939-8582-1
Preparation of anti-miR PNAs for drug development and nanomedicine / Manicardi, Alex; Gambari, Roberto; de Cola, Luisa; Corradini, Roberto. - STAMPA. - 1811:(2018), pp. 49-63. [10.1007/978-1-4939-8582-1_4]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2848674
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