Innovative and highly performing smart voltammetric immunosensors for rapid and effective serological tests aimed at the determination of SARS-CoV-2 antibodies were developed and validated in human serum matrix. Two immunosensors were developed for the determination of immunoglobulins directed against either the nucleocapsid or the spike viral antigen proteins. The immunosensors were realized using disposable screen-printed electrodes modified with nanostructured materials for the immobilization of the antigens. Fast quantitative detection was achieved, with analysis duration being around 1 h. Signal readout was carried out through a smart, compact and battery-powered potentiostat, based on aWi-Fi protocol and devised for the Internet of Things (IoT) paradigm. This device is used for the acquisition, storage and sharing of clinical data. Outstanding immunosensors’ sensitivity, specificity and accuracy (100%) were assessed, according to the diagnostic guidelines for epidemiological data. The overall performance of the sensing devices, combined with the portability of the IoT-based device, enables their suitability as a high-throughput diagnostic tool. Both of the immunosensors were validated using clinical human serum specimens from SARS-CoV-2 infected patients, provided by IRCCS Ospedale San Raffaele.

Smart Immunosensors for Point-of-Care Serological Tests Aimed at Assessing Natural or Vaccine-Induced SARS-CoV-2 Immunity / Fortunati, Simone; Giannetto, Marco; Giliberti, Chiara; Bolchi, Angelo; Ferrari, Davide; Locatelli, Massimo; Bianchi, Valentina; Boni, Andrea; De Munari, Ilaria; Careri, Maria. - In: SENSORS. - ISSN 1424-8220. - 22:14(2022). [10.3390/s22145463]

Smart Immunosensors for Point-of-Care Serological Tests Aimed at Assessing Natural or Vaccine-Induced SARS-CoV-2 Immunity

Fortunati, Simone
Investigation
;
Giannetto, Marco
Writing – Original Draft Preparation
;
Giliberti, Chiara
Investigation
;
Bolchi, Angelo
Resources
;
Ferrari, Davide
Resources
;
Bianchi, Valentina
Data Curation
;
Boni, Andrea
Methodology
;
De Munari, Ilaria
Supervision
;
Careri, Maria
Project Administration
2022

Abstract

Innovative and highly performing smart voltammetric immunosensors for rapid and effective serological tests aimed at the determination of SARS-CoV-2 antibodies were developed and validated in human serum matrix. Two immunosensors were developed for the determination of immunoglobulins directed against either the nucleocapsid or the spike viral antigen proteins. The immunosensors were realized using disposable screen-printed electrodes modified with nanostructured materials for the immobilization of the antigens. Fast quantitative detection was achieved, with analysis duration being around 1 h. Signal readout was carried out through a smart, compact and battery-powered potentiostat, based on aWi-Fi protocol and devised for the Internet of Things (IoT) paradigm. This device is used for the acquisition, storage and sharing of clinical data. Outstanding immunosensors’ sensitivity, specificity and accuracy (100%) were assessed, according to the diagnostic guidelines for epidemiological data. The overall performance of the sensing devices, combined with the portability of the IoT-based device, enables their suitability as a high-throughput diagnostic tool. Both of the immunosensors were validated using clinical human serum specimens from SARS-CoV-2 infected patients, provided by IRCCS Ospedale San Raffaele.
Smart Immunosensors for Point-of-Care Serological Tests Aimed at Assessing Natural or Vaccine-Induced SARS-CoV-2 Immunity / Fortunati, Simone; Giannetto, Marco; Giliberti, Chiara; Bolchi, Angelo; Ferrari, Davide; Locatelli, Massimo; Bianchi, Valentina; Boni, Andrea; De Munari, Ilaria; Careri, Maria. - In: SENSORS. - ISSN 1424-8220. - 22:14(2022). [10.3390/s22145463]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2927331
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