Two nickel(II) complexes with substituted bipyridine ligand of the type [Ni(NN)3](ClO4)2, where NN is 4,4'-dimethyl-2,2'-bipyridine (dimethylbpy) (1) and 4,4'-dimethoxy-2,2'-bipyridine (dimethoxybpy) (2) have been synthesized, characterized, and their interaction with DNA and BSA studied by different physical methods. X-ray crystal structure of 1 shows a six-coordinate complex in a distorted octahedral geometry. DNA binding studies of 1 and 2 reveal that both complexes sit in DNA groove and then interact with neighboring nucleotides differently; 2 undergoes a partial intercalation. This is supprted by molecular docking studies, where hydrophobic interactions are aparent between 1 and DNA as compared to, hydrogen bonding, hydrophobic, and π-π interactions between 2 and DNA minor groove. Moreover, the two complexes exhibit oxidative cleavage of supercoiled plasmid DNA in the presence of hydrogen peroxide as an activator in the order of 1 > 2. In terms of interaction with BSA, the results of spectroscopic methods and molecular docking show that 1 binds with BSA only via hydrophobic contacts while 2 interacts through hydrophobic and hydrogen bonding. It has been extensively demonstrated that the nature of the methyl- and methoxy-groups in ligands is a strong determinant of the bioactivity of nickel(II) complexes. This may justify the above differences in biomolecular interactions. In addition, the in vitro cytotoxicity of the complexes on human carcinoma cells lines (MCF-7, HT-29, and U-87) has been examined by MTT assay. According to our observations, 1 and 2 display cytotoxicity activity against selected cell lines.

Tris-chelated complexes of nickel(II) with bipyridine derivatives: DNA binding and cleavage, BSA binding, molecular docking, and cytotoxicity / Anjomshoa, Marzieh; Torkzadeh-Mahani, Masoud; Sahihi, Mehdi; Rizzoli, Corrado; Ansari, Mehdi; Janczak, Jan; Sherafat Esfahani, Sheila; Ataei, Farangis; Dehkhodaei, Monireh; Amirheidari, Bagher. - In: JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS. - ISSN 0739-1102. - 37:15(2019), pp. 3887-3904. [10.1080/07391102.2018.1534700]

Tris-chelated complexes of nickel(II) with bipyridine derivatives: DNA binding and cleavage, BSA binding, molecular docking, and cytotoxicity

Rizzoli, Corrado;
2019-01-01

Abstract

Two nickel(II) complexes with substituted bipyridine ligand of the type [Ni(NN)3](ClO4)2, where NN is 4,4'-dimethyl-2,2'-bipyridine (dimethylbpy) (1) and 4,4'-dimethoxy-2,2'-bipyridine (dimethoxybpy) (2) have been synthesized, characterized, and their interaction with DNA and BSA studied by different physical methods. X-ray crystal structure of 1 shows a six-coordinate complex in a distorted octahedral geometry. DNA binding studies of 1 and 2 reveal that both complexes sit in DNA groove and then interact with neighboring nucleotides differently; 2 undergoes a partial intercalation. This is supprted by molecular docking studies, where hydrophobic interactions are aparent between 1 and DNA as compared to, hydrogen bonding, hydrophobic, and π-π interactions between 2 and DNA minor groove. Moreover, the two complexes exhibit oxidative cleavage of supercoiled plasmid DNA in the presence of hydrogen peroxide as an activator in the order of 1 > 2. In terms of interaction with BSA, the results of spectroscopic methods and molecular docking show that 1 binds with BSA only via hydrophobic contacts while 2 interacts through hydrophobic and hydrogen bonding. It has been extensively demonstrated that the nature of the methyl- and methoxy-groups in ligands is a strong determinant of the bioactivity of nickel(II) complexes. This may justify the above differences in biomolecular interactions. In addition, the in vitro cytotoxicity of the complexes on human carcinoma cells lines (MCF-7, HT-29, and U-87) has been examined by MTT assay. According to our observations, 1 and 2 display cytotoxicity activity against selected cell lines.
2019
Tris-chelated complexes of nickel(II) with bipyridine derivatives: DNA binding and cleavage, BSA binding, molecular docking, and cytotoxicity / Anjomshoa, Marzieh; Torkzadeh-Mahani, Masoud; Sahihi, Mehdi; Rizzoli, Corrado; Ansari, Mehdi; Janczak, Jan; Sherafat Esfahani, Sheila; Ataei, Farangis; Dehkhodaei, Monireh; Amirheidari, Bagher. - In: JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS. - ISSN 0739-1102. - 37:15(2019), pp. 3887-3904. [10.1080/07391102.2018.1534700]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2853730
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