Pseudomonas syringae pv. tomato (Pst) is the causal agent of bacterial speck disease in tomato. Resistance to Pst is determined by Pto, a single resistance gene that belongs to a multi-gene family clustered on chromosome 5. Pst-resistant phenotypes in cultivated tomato are determined by a semi-dominant allele of Solanum pimpinellifolium, which was introgressed into Solanum lycopersicum in the past century. Seed companies, which are continuously interested in producing resistant varieties, can benefit from genetic markers closely linked to the Pto locus in breeding programs based on marker-assisted selection. In this research, three sequence characterised amplified region markers have been developed for identification of resistant and susceptible genotypes of Solanum lycopersicum. A cleaved amplified polymorphic sequence marker has been adapted to a real-time polymerase chain reaction platform using high-resolution melting curve analysis. Application to a tomato population for breeding programs is described. Advantages and disadvantages of the different markers are discussed.

Determining resistance to Pseudomonas syringae in tomato, a comparison with different molecular markers / I., Orsi; Malatrasi, Marina; E., Belfanti; Gulli', Mariolina; Marmiroli, Nelson. - In: MOLECULAR BREEDING. - ISSN 1380-3743. - 30:2(2012), pp. 967-974. [10.1007/s11032-011-9681-8]

Determining resistance to Pseudomonas syringae in tomato, a comparison with different molecular markers

MALATRASI, Marina;GULLI', Mariolina;MARMIROLI, Nelson
2012-01-01

Abstract

Pseudomonas syringae pv. tomato (Pst) is the causal agent of bacterial speck disease in tomato. Resistance to Pst is determined by Pto, a single resistance gene that belongs to a multi-gene family clustered on chromosome 5. Pst-resistant phenotypes in cultivated tomato are determined by a semi-dominant allele of Solanum pimpinellifolium, which was introgressed into Solanum lycopersicum in the past century. Seed companies, which are continuously interested in producing resistant varieties, can benefit from genetic markers closely linked to the Pto locus in breeding programs based on marker-assisted selection. In this research, three sequence characterised amplified region markers have been developed for identification of resistant and susceptible genotypes of Solanum lycopersicum. A cleaved amplified polymorphic sequence marker has been adapted to a real-time polymerase chain reaction platform using high-resolution melting curve analysis. Application to a tomato population for breeding programs is described. Advantages and disadvantages of the different markers are discussed.
2012
Determining resistance to Pseudomonas syringae in tomato, a comparison with different molecular markers / I., Orsi; Malatrasi, Marina; E., Belfanti; Gulli', Mariolina; Marmiroli, Nelson. - In: MOLECULAR BREEDING. - ISSN 1380-3743. - 30:2(2012), pp. 967-974. [10.1007/s11032-011-9681-8]
File in questo prodotto:
File Dimensione Formato  
Abs MOLECULAR BREEDING.pdf

non disponibili

Tipologia: Abstract
Licenza: Creative commons
Dimensione 79.3 kB
Formato Adobe PDF
79.3 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Molecular Breeding.pdf

non disponibili

Tipologia: Documento in Pre-print
Licenza: Creative commons
Dimensione 316.88 kB
Formato Adobe PDF
316.88 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2357084
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 3
  • ???jsp.display-item.citation.isi??? 2
social impact