Abstract Aging is accompanied by a complex dynamics of CD8þ T cell subsets whose origin is unclear. To evaluate the impact of Epstein-Barr virus (EBV) and cytomegalovirus (CMV) chronic infections on CD8þ T cells in far advanced age, we studied CD8þ T cells frequencies and phenotype in nonagenarians and centenarians by HLA-A*0201- and HLA-B*0702-tetramers incorporating epitopes specific of both viruses along with viral replication. The results demonstrate that EBV and CMV infections induce quantitatively and qualitatively different CD8þ Tcell responses in advanced aging. The frequency and absolute number of CD8þ T cells specific for one lytic and two latent EBV-epitopes, were relatively low and mostly included within CD8þ CD28þ cells. By contrast, CMV infection was characterized by highly variable numbers of CD8þ T cells specific for two differently restricted CMV-epitopes that, in some subjects, were strikingly expanded. Moreover, the great majority of anti-CMV CD8þ T cells did not bear CD28 antigen. Notwithstanding the expansion of CMV-specific CD8þ lymphocytes, CMV-DNA detection in blood samples was invariably negative. Altogether, we suggest that CMV, but not EBV, can sustain chronic activation of the HLA-class I restricted effector arm in elderly that might have detrimental effects on age-associated diseases.

Different contribution of EBV and CMV infections in very long-term carriers to age-related alterations of CD8+ T cells / Vescovini, Rosanna; Telera, A.; Fagnoni, F. F.; Biasini, C.; Medici, Maria Cristina; Valcavi, PIER PAOLO; DI PEDE, P.; Lucchini, G.; Zanlari, L.; Passeri, Giovanni; Zanni, F.; Chezzi, Carlo; Franceschi, C.; Sansoni, Paolo. - In: EXPERIMENTAL GERONTOLOGY. - ISSN 0531-5565. - 39(8):(2004), pp. 1233-1243. [10.1016/j.exger.2004.04.004]

Different contribution of EBV and CMV infections in very long-term carriers to age-related alterations of CD8+ T cells

VESCOVINI, Rosanna;MEDICI, Maria Cristina;VALCAVI, PIER PAOLO;PASSERI, Giovanni;CHEZZI, Carlo;SANSONI, Paolo
2004-01-01

Abstract

Abstract Aging is accompanied by a complex dynamics of CD8þ T cell subsets whose origin is unclear. To evaluate the impact of Epstein-Barr virus (EBV) and cytomegalovirus (CMV) chronic infections on CD8þ T cells in far advanced age, we studied CD8þ T cells frequencies and phenotype in nonagenarians and centenarians by HLA-A*0201- and HLA-B*0702-tetramers incorporating epitopes specific of both viruses along with viral replication. The results demonstrate that EBV and CMV infections induce quantitatively and qualitatively different CD8þ Tcell responses in advanced aging. The frequency and absolute number of CD8þ T cells specific for one lytic and two latent EBV-epitopes, were relatively low and mostly included within CD8þ CD28þ cells. By contrast, CMV infection was characterized by highly variable numbers of CD8þ T cells specific for two differently restricted CMV-epitopes that, in some subjects, were strikingly expanded. Moreover, the great majority of anti-CMV CD8þ T cells did not bear CD28 antigen. Notwithstanding the expansion of CMV-specific CD8þ lymphocytes, CMV-DNA detection in blood samples was invariably negative. Altogether, we suggest that CMV, but not EBV, can sustain chronic activation of the HLA-class I restricted effector arm in elderly that might have detrimental effects on age-associated diseases.
2004
Different contribution of EBV and CMV infections in very long-term carriers to age-related alterations of CD8+ T cells / Vescovini, Rosanna; Telera, A.; Fagnoni, F. F.; Biasini, C.; Medici, Maria Cristina; Valcavi, PIER PAOLO; DI PEDE, P.; Lucchini, G.; Zanlari, L.; Passeri, Giovanni; Zanni, F.; Chezzi, Carlo; Franceschi, C.; Sansoni, Paolo. - In: EXPERIMENTAL GERONTOLOGY. - ISSN 0531-5565. - 39(8):(2004), pp. 1233-1243. [10.1016/j.exger.2004.04.004]
File in questo prodotto:
File Dimensione Formato  
Sansoni Exp Gerontol 2004.pdf

non disponibili

Tipologia: Documento in Post-print
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 282.71 kB
Formato Adobe PDF
282.71 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/1440624
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 98
  • ???jsp.display-item.citation.isi??? 87
social impact